Morphological, histological and molecular characterization of three Myxobolus species (Cnidaria: Myxosporea) from silver carp Hypophthalmichthys molitrix Valenciennes and bighead carp Hypophthalmichthys nobilis Richardson in China.
Morphological, histological and molecular characterization of three Myxobolus species (Cnidaria: Myxosporea) from silver carp Hypophthalmichthys molitrix Valenciennes and bighead carp Hypophthalmichthys nobilis Richardson in China.
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DOI：
10.1016/j.parint.2018.04.011
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发表时间：
2018-08
影响因子：
1.9
通讯作者：
Bo Zhang;Z. Gu;Yang Liu
中科院分区：
文献类型：
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作者：
Bo Zhang;Z. Gu;Yang Liu
从我国白鲢和鳙鱼中分离得到3个粘鱼种。在本研究中，我们通过形态学、组织学和分子学方法补充了它们的分类学特征。Chen et Ma，1998中的Myxobolus kiucowensis Chen在鳙鱼的肠壁中形成了小的椭圆形疟原虫。其孢子正面观呈不对称倒卵形，侧面观呈梭形。在一些孢子的前部观察到微小的乳头突出。两个梨形极性蒴果不等长。组织学上，M.九州文丝虫感染宿主肠道的肌层。Myxobolus abitusLi et Nie, 1973 在鲢鱼的鳃中形成香肠状疟原虫。其孢子正面观呈扁圆形，侧面观呈纺锤形。两个梨形极囊不等长，囊间有明显的阑尾。组织学检查显示M.宿主鳃的层间上皮细胞中发育有能力。巴甫洛夫斯基粘液菌(Akhmerov, 1954) Landsberg et Lom, 1991 在鲢鱼和鳙鱼的鳃中形成香肠样疟原虫。 M.孢子巴甫洛夫斯基在正面观察时呈近似椭圆形，在侧面观察时呈梭形。两个梨形极性蒴果不等长。 BLAST搜索表明M的SSU rDNA序列。九州文西斯和 M. abitus 与任何序列都不相同，但是 M 的 SSU rDNA 序列。巴甫洛夫斯基与M的相同。巴甫洛夫斯基之前录制过。系统发育分析表明，目前的三个物种在鲤科和亚洲组中稳健地聚集在一起。
ThreeMyxobolusspecies were obtained from silver carpHypophthalmichthys molitrixValenciennes and bighead carpHypophthalmichthys nobilisRichardson in China. In the present study, we supplemented their taxonomic characteristics by the morphological, histological and molecular methods.Myxobolus kiuchowensisChen in Chen et Ma, 1998 formed small ellipsoidal plasmodia in the intestinal wall of bighead carp. Its spores appeared asymmetrical obovate in frontal view and fusiform in lateral view. Tiny mamillary protrusion in the anterior of some spores was observed. Two pyriform polar capsules were unequal. Histologically,M. kiuchowensisinfected the tunica muscularis of host intestine.Myxobolus abitusLi et Nie, 1973 formed sausage–like plasmodia in the gills of silver carp. Its spores appeared oblate in frontal view and fusiform in lateral view. Two pyriform polar capsules were unequal and an obvious inter–capsule appendix was observed. Histological examination revealed thatM. abitusdeveloped in the interlamellar–epithelium of host gills.Myxobolus pavlovskii(Akhmerov, 1954) Landsberg et Lom, 1991 formed sausage–like plasmodia both in the gills of silver carp and bighead carp. Spores ofM. pavlovskiiwere proximate oval in frontal view and fusiform in lateral view. Two pyriform polar capsules were unequal. The BLAST search indicated the SSU rDNA sequences ofM. kiuchowensisandM. abituswere not identical to any sequence, however, the SSU rDNA sequences ofM. pavlovskiiwere identical to that ofM. pavlovskiirecorded previously. Phylogenetic analysis showed that the present three species robustly clustered together in Cyprinid group and Asia group.