Determinants of kinesin motor polarity
Determinants of kinesin motor polarity
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DOI：
10.1126/science.281.5380.1200
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发表时间：
1998-08-21
期刊：
影响因子：
56.9
通讯作者：
Waligora, KW
中科院分区：
文献类型：
--
作者：
Endow, SA;Waligora, KW
驱动蛋白运动蛋白家族成员以特征极性沿沿着微管运动。嵌合电机包含柄和颈部的负端导向电机，Ncd，融合到电机域的正端导向驱动蛋白进行了分析。Ncd柄和颈部逆转了驱动蛋白运动的极性，但Ncd颈部的突变使嵌合运动恢复为正末端运动。因此，残基或区域有助于电机极性必须存在于NCD颈部和驱动蛋白电机核心。颈部运动交界处是关键的Ncd负端运动，附件的颈部柄也可能发挥作用。
The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.