Cooperation of calcineurin and ERK for UTP-induced IL-6 production in HaCaT keratinocytes
Cooperation of calcineurin and ERK for UTP-induced IL-6 production in HaCaT keratinocytes
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DOI：
10.1016/j.ejphar.2007.07.058
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发表时间：
2007-11-14
影响因子：
5
通讯作者：
Nakahata, Norimichi
中科院分区：
文献类型：
--
作者：
Kobayashi, Daisaku;Ohkubo, Satoko;Nakahata, Norimichi
UTP导致HaCaT角质形成细胞中IL-6的产生，其部分地被有丝分裂原活化蛋白激酶激酶（MEK）抑制剂PD 98059抑制，这表明除了细胞外信号调节激酶（ERK）途径之外的途径参与产生。在本研究中，我们研究了钙调神经磷酸酶参与UTP诱导的HaCaT角质形成细胞产生白细胞介素（IL）-6。钙调磷酸酶抑制剂FK 506和环孢素A部分抑制UTP诱导的IL-6 mRNA表达和蛋白质产生。此外，FK 506和PD 98059联合应用可协同抑制UTP诱导的IL-6产生。这些结果表明ERK和钙调神经磷酸酶协同参与UTP诱导的IL-6产生。(c)2007 Elsevier B. V.保留所有权利。
UTP causes IL-6 production in HaCaT keratinocytes, which is partially inhibited by PD98059, a mitogen-activated protein kinase kinase (MEK) inhibitor, suggesting that a pathway other than the extracellular signal-regulated kinase (ERK) pathway is involved in the production. In the present study, we examined the involvement of calcineurin in the UTP-induced interleukin (IL)-6 production in HaCaT keratinocytes. FK506 and cyclosporine A, calcineurin inhibitors, partially inhibited UTP-induced IL-6 mRNA expression and protein production. In addition, combined application of FK506 and PD98059 synergistically inhibited the UTP-induced IL-6 production. These results suggest that ERK and calcineurin are cooperatively involved in UTP-induced IL-6 production. (c) 2007 Elsevier B.V. All rights reserved.