PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.
PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.
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PPP5C 通过调节 JNK 和 ERK1/2 磷酸化促进人前列腺癌的细胞增殖和存活。
DOI：
10.2147/ott.s161280
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发表时间：
2018
影响因子：
4
通讯作者：
Xu DF
中科院分区：
文献类型：
--
作者：
Lv JM;Chen L;Gao Y;Huang H;Pan XW;Liu X;Chen M;Qu FJ;Li L;Wang JK;Cui XG;Xu DF
前列腺癌（PCa）是最常见的恶性肿瘤之一，也是男性癌症相关死亡的主要原因。因此，有必要探索新的分子靶点，以提高PCa的诊断和治疗水平。丝氨酸/苏氨酸蛋白磷酸酶5（PPP 5C）是一种参与细胞复杂生理活动的重要分子。本研究旨在检测PPP 5C在前列腺癌组织中的表达水平，进一步探讨PPP 5C在前列腺癌中的生物学功能及作用机制。PPP 5C的表达水平通过免疫组织化学和ONCOM-INE数据集进行分析。构建慢病毒介导的短发夹状RNA（shRNA）沉默前列腺癌细胞PPP 5C的表达。采用MTT法和集落形成法检测细胞活力和增殖能力，流式细胞仪检测细胞周期和凋亡情况。Western blot检测下游蛋白水平和蛋白磷酸化水平的变化。PPP 5C在PCa组织中高度表达，通过免疫组织化学和ONCOMINE数据集分析。PPP 5C敲低抑制PCa细胞中的细胞增殖和集落形成。流式细胞仪分析显示，去除PPP 5C后的DU 145、PC 3和22 RV 1 PCa细胞均被阻滞于G 0/G1期，并发生凋亡。Western blot分析表明PPP 5C基因敲低可促进JNK和ERK的磷酸化。PPP 5C有可能成为前列腺癌新的诊断标志物和治疗靶点。
Prostate cancer (PCa) is one of the most common malignancies and a major leading cause of cancer-related deaths in males. And it is necessary to explore new molecular targets to enhance diagnosis and treatment level of the PCa. Serine/threonine protein phosphatase 5 (PPP5C) is a vital molecule that Involve in complex cell physiological activity. The objective of this study was to detecte the expression level of PPP5C in the tissue of prostate cancer patients and further discussed the PPP5C biological function and mechanisms on the PCa. The expression level of PPP5C was analyzed by immunohistochemistry and ONCOM-INE datasets. Lentivirus-mediated short hairpin RNA (shRNA) was constructed to silence the expression of PPP5C in prostate cancer cell. Cell viability and proliferation were measured using MTT and colony formation, and the cell cycle and apoptosis was analyszed by flow cytometry. The changes of downstream protein level and protein phosphorylation level were detected by western blot. PPP5C was highly expressed in PCa tissue as analyzed by immunohistochemistry and ONCOMINE datasets. PPP5C Knockdown inhibited cell proliferation and colony formation in PCa cells. Flow cytometry analysis showed that DU145, PC3 and 22RV1 PCa cells deprived of PPP5C were arrested in G0/G1 phase and became apoptotic. Western blot analysis indicated that PPP5C knockdown could promote JNK and ERK phosphorylation. Our study indicated that the PPP5C may become a new potential diagnostic biomarker and therapeutic target for the PCa.