Immobilization antigens provide orange-spotted grouper (Epinephelus coioides) protection against Cryptocaryon irritans infection
Immobilization antigens provide orange-spotted grouper (Epinephelus coioides) protection against Cryptocaryon irritans infection
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DOI：
10.1016/j.aquaculture.2023.739722
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发表时间：
2023-10
期刊：
影响因子：
4.5
通讯作者：
Xiong Li;Hongping Chen;Yihao Cen;Jiule Wang;Na Ni;X. Dan;Z. Mo;Yanwei Li
中科院分区：
文献类型：
--
作者：
Xiong Li;Hongping Chen;Yihao Cen;Jiule Wang;Na Ni;X. Dan;Z. Mo;Yanwei Li
疫苗接种是预防隐核虫刺激爆发的一种方法，而固定化抗原（i抗原）被认为是针对寄生虫的疫苗开发的一种方法。然而，c的信息。刺激性抗原是有限的。在这项研究中，我们从mc中鉴定了3个固定抗原基因（CI-IA1， CI-IA2和CI-IA8）。irritans。这三种i抗原都含有信号肽、跨膜结构域和GPI锚点。在i抗原序列中发现保守的抗原表位位点。在去除信号肽和跨膜结构域后，对截断i抗原的密码子进行优化，并在大肠杆菌中表达。经表面暴露或腹腔注射抗原产生的石斑鱼抗血清均能识别重组i抗原，表明重组i抗原具有免疫原性。经腹腔注射重组i抗原后，发现接种CI-IA1、CI-IA2和CI-IA8可产生较高的固定化效价，保护石斑鱼免受c感染。irritansinfection。该寄生虫对Ci-IA1、CI-IA2和CI-IA8的抑制率分别为25.55%、17.13%和21.05%。在疫苗组的血清和黏液中检测到较高的特异性IgM抗体滴度。确定免疫接种后免疫相关基因的调控，表明免疫接种过程中适应性免疫反应活跃。这些结果表明，接种重组Ci-IA1、CI-IA2和CI-IA8可引起石斑鱼对c的保护性免疫。刺激物和这些i抗原可作为疫苗开发的潜在抗原。
Vaccination is a way to preventCryptocaryon irritansoutbreaks and immobilization antigens (I-antigens) are considered for vaccine development against the parasite.However, the information ofC. irritansI-antigens is limit. In this study, we characterized three immobilization antigen genes (CI-IA1, CI-IA2, and CI-IA8) fromC. irritans. All three I-antigens contain signal peptides, transmembrane domains, and a GPI anchor. Conserved antigenic epitope sites were found in the I-antigens sequences. After removing signal peptides and transmembrane domains, the codons of truncated I-antigens were optimized and expressed inEscherichia coli. The purified recombinant I-antigens were recognized by grouper anti-serum generated by surface exposure or intraperitoneal injection with theronts, indicating that the recombinant I-antigens have immunogenicity. After intraperitoneal injection with recombinant I-antigens, it was found that vaccination with CI-IA1, CI-IA2, and CI-IA8 generated higher immobilization titer and protected grouper againstC. irritansinfection. The parasite reduced the rate of Ci-IA1, CI-IA2, and CI-IA8 by 25.55%, 17.13%, and 21.05%, respectively. Higher specific IgM antibody titers were detected in serum and mucus from the vaccine group. The regulation of immune-related genes after the vaccination was determined and showed that the adaptive immune response was active during the immunization. These findings suggested that vaccination with recombinant Ci-IA1, CI-IA2, and CI-IA8 elicits protective immunity in grouper againstC. irritansand those I-antigens may serve as a potential antigen for vaccine development.