GENERATION OF SUPEROXIDE ANION BY THE NADH DEHYDROGENASE OF BOVINE HEART-MITOCHONDRIA
GENERATION OF SUPEROXIDE ANION BY THE NADH DEHYDROGENASE OF BOVINE HEART-MITOCHONDRIA
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DOI：
10.1042/bj1910421
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发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
BOVERIS, A
中科院分区：
文献类型：
--
作者：
TURRENS, JF;BOVERIS, A
来自牛心脏的亚线粒体颗粒，其中通过添加NADH和鱼藤酮或通过反向电子转移还原NADH脱氢酶，产生0.9 ±。0.1 nmol O2-/min/mg蛋白质，pH 7.4和30 ℃。C.当以NADH为底物时，鱼藤酮、抗霉素和氰化物增加O2-的产生。在补充NADH和抗霉素的亚线粒体颗粒中，鱼藤酮具有双相效应：它增加NADH脱氢酶的O2-产生，并抑制泛醌-细胞色素B位点的O2-产生。生成的O2-的NADH脱氢酶支持的反向电子转移被抑制鱼藤酮，解偶联剂羰基氰化物p-三氟甲氧基苯腙和寡霉素的浓度类似于那些需要抑制能量依赖性琥珀酸-NAD还原酶。氰化物不影响在NADH脱氢酶的O2-的产生，但抑制在泛醌-细胞色素B网站的O2-的生产。在pH 7.4时，NADH脱氢酶产生的O2-约为泛醌-细胞色素B区域产生的O2-的50%。在pH范围从7.0至8.8的2线粒体O2-生成的网站的添加剂进行了观察。描述了一种依赖于O2的自催化过程，该过程需要NADH、亚线粒体颗粒和肾上腺素。
Submitochondrial particles from bovine heart in which NADH dehydrogenase is reduced by either addition of NADH and rotenone or by reversed electron transfer generate 0.9 .+-. 0.1 nmol of O2-/min/mg of protein at pH 7.4 and at 30.degree. C. When NADH is used as substrate, rotenone, antimycin and cyanide increase O2- production. In NADH- and antimycin-supplemented submitochondrial particles, rotenone has a biphasic effect: it increases O2- production at the NADH dehydrogenase and inhibits O2- production at the ubiquinone-cytochrome b site. Generation of O2- by the NADH dehydrogenase supported by reversed electron transfer was inhibited by rotenone, the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone and oligomycin at concentrations similar to those required to inhibit energy-dependent succinate-NAD reductase. Cyanide did not affect O2- generation at the NADH dehydrogenase, but inhibited O2- production at the ubiquinone-cytochrome b site. Production of O2- at the NADH dehydrogenase is about 50% of the O2- generation at the ubiquinone-cytochrome b area at pH 7.4. Additivity of the 2 mitochondrial sites of O2- generation was observed over the pH range from 7.0 to 8.8. An O2--dependent autocatalytic process that requires NADH, submitochondrial particles and adrenaline [epinephrine] is described.