[X-ray irradiation against Echinococcus multilocularis protoscoleces in vitro].
[X-ray irradiation against Echinococcus multilocularis protoscoleces in vitro].
复制标题
DOI：
--
复制
发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Y. Bao;R. Mao;Hong-zhi Qi;Yue-fen Zhang;Ya-qiong Ni;Zengru Xie;Tursun Aziguli;H. Wen
中科院分区：
文献类型：
--
作者：
Y. Bao;R. Mao;Hong-zhi Qi;Yue-fen Zhang;Ya-qiong Ni;Zengru Xie;Tursun Aziguli;H. Wen
目的探讨X线照射对多房棘球绦虫原孢子的影响。方法从感染多房棘球蚴的沙鼠包囊中采集原虫卵，用RPMI 1640培养基培养。在培养3天后，将原球茎以约10（4）个/瓶的密度分装到培养瓶中。每组有10个培养瓶。设空白对照组、低剂量组、空白对照组、低剂量组、空白对照组、空白对照组、低剂量组、空白对照组、空白对照组、低剂量组、空白对照组、空白对照组、低剂量组、空白对照组（15戈伊和30戈伊），中剂量组（45戈伊和60戈伊），高剂量组阿苯达唑组：75戈伊和90戈伊45戈伊X线+2 500 ng/ml阿苯达唑组; 75戈伊X线+ 2 500 ng/ml阿苯达唑组。在培养3天后，每隔一天以100 cm的源-皮肤距离和200 cGy/min的剂量率对原核细胞进行三次照射。照射后每天用0.1%伊红染色法在光镜下计数原虫，计算原虫死亡率（每100个原虫），直至实验组原虫全部死亡。同时观察原蜱的形态学变化。结果X线组与空白对照组、X线+阿苯达唑组与阿苯达唑组原头节死亡率差异有统计学意义（P < 0.05）。阿苯达唑组原头节死亡率高于空白对照组（P < 0.05）。阿苯达唑联合放疗组与单纯放疗组的原头节死亡率差异有显著性（P < 0.05）。照射前原细胞形态正常，结构完整。照射后，寄生虫多为外翻型原虫卵，钩头紊乱，髋臼变形，最后死亡。结论X射线在体外对泡球蚴有杀灭作用。
OBJECTIVE To explore the effect of X-ray irradiation on Echinococcus multilocularis protoscoleces in vitro. METHODS Echinococcus multilocularis protoscoleces were collected from cysts of infected Meriones meridianus and then cultured in RPMI 1640 medium. Protoscoleces were subpackaged into culture flasks at a density of about 10(4) per flask after culture for 3 days. Each group has 10 culture flasks. There were seven groups named as blank control group, low dose group (15 Gy and 30 Gy), medium dose group (45 Gy and 60 Gy), high dose group (75 Gy and 90 Gy), albendazole group (2 500 ng/ml), 45 Gy X-ray + 2 500 ng/ml albendazole group, and 75 Gy X-ray + 2 500 ng/ml albendazole group. Protoscoleces received three radiations on every other day with a source-skin distance of 100 cm and at a dose rate of 200 cGy/min after 3 days in culture. At each day after irradiation, protoscoleces were counted by light microscope with 0.1% eosin staining, and calculated mortality rate (per 100 protoscoleces) until all the parasites in experimental groups died. At the same time, the morphological changes of protoscoleces were observed. RESULTS There were significant differences in protoscolex mortality between X-ray groups and blank control group (P < 0.05), between X-ray + albendazole groups and albendazole group (P < 0.05). Protoscolex mortality in albendazole group were higher than that of blank control group (P < 0.05). Significant difference were also found in protoscolex mortality between albendazole combined with radiation and radiation only (P < 0.05). Before radiation, protoscoleces was normal with complete structure. After radiation, the parasites were mostly valgus type protoscoleces with disordered rostellar hooks and deformed acetabulum, and finally died. CONCLUSION X-ray can kill Echinococcus multilocularis protoscoleces in vitro.