Tandem mass spectrometry of intact proteins for characterization of biomarkers from Bacillus cereus T spores.
Tandem mass spectrometry of intact proteins for characterization of biomarkers from Bacillus cereus T spores.
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对完整蛋白质进行串联质谱分析，以表征蜡状芽孢杆菌 T 孢子的生物标志物。
DOI：
10.1021/ac010672n
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发表时间：
2001
影响因子：
7.4
通讯作者：
Catherine Fenselau
中科院分区：
文献类型：
--
作者：
P. Demirev;Javier Ramirez;Catherine Fenselau
采用高分辨率串联傅里叶变换离子回旋共振质谱法对蜡状芽孢杆菌T孢子中的完整蛋白质生物标志物进行了分析。两种技术已被应用于激发的孤立的多电荷的前体离子物种：持续的非共振辐射/碰撞活化解离和电子捕获解离。片段衍生的序列标签和BLAST序列相似性蛋白质组数据库搜索允许以前所未有的特异性明确鉴定主要生物标志物蛋白。序列特异性片段化模式进一步证实了蛋白质鉴定。此外，方法学结合完整蛋白质的精确质量测量与蛋白质组数据库中包含的额外信息允许从B分离的几种其他蛋白质生物标志物的暂定分配。蜡状T孢子。我们认为，涉及蛋白质生物标志物串联质谱的方法，结合生物信息学，可以大大提高单个微生物鉴定的特异性，特别是在复杂的环境中。
Intact protein biomarkers from Bacillus cereus T spores have been analyzed by high-resolution tandem Fourier transform ion cyclotron resonance mass spectrometry. Two techniques have been applied for excitation of the isolated multiply charged precursor ion species: sustained off-resonance irradiation/collisionally activated dissociation and electron capture dissociation. Fragmentation-derived sequence tags and BLAST sequence similarity proteome database searches allow unequivocal identification of the major biomarker protein with unprecedented specificity. Sequence-specific fragmentation patterns further confirm protein identification. Moreover, methodology combining accurate mass measurements of intact proteins with additional information contained in a proteome database permits tentative assignment of several other protein biomarkers isolated from the B. cereus T spores. We argue that approaches involving tandem MS of protein biomarkers, combined with bioinformatics, can drastically improve the specificity of individual microorganism identification, particularly in complex environments.