Boundary-activated dissociation of peptide ions in a quadrupole ion trap.
Boundary-activated dissociation of peptide ions in a quadrupole ion trap.
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DOI：
10.1021/ac970579s
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发表时间：
1998-01
影响因子：
7.4
通讯作者：
R. Vachet;G. Glish
中科院分区：
文献类型：
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作者：
R. Vachet;G. Glish
多肽的边界激活解离(BAD)已被研究作为一种替代共振激发的方法来实现四极离子陷阱中的碰撞诱导解离。BAD的非共振激发机制克服了共振激发的一个主要缺点，即共振激发频率随离子空间电荷的变化。与共振激发一样，重气体(Ar、Xe)的脉冲引入扩展了串联质谱仪对多肽离子进行BAD的适用范围。离子活化过程中重气体的存在允许更大的内能沉积，也使BAD能够在比以前报道的这项技术更低的俘获场强(较低的Q值)下进行。这扩大了产品离子可以收集的质量范围。
Boundary-activated dissociation (BAD) of peptides has been investigated as an alternative to the use of resonant excitation to effect collision-induced dissociation in the quadrupole ion trap. BAD's nonresonant excitation mechanism overcomes a major drawback in resonant excitation, namely, the variation of the resonant excitation frequency as a function of ion space charging. As with resonant excitation, the pulsed introduction of heavy gases (argon, xenon) extends the applicability of BAD when tandem mass spectrometry is performed on peptide ions. The presence of heavy gases during ion activation allows greater internal energy deposition and also enables BAD to be performed at much lower trapping field strengths (lower q values) than previously reported for this technique. This extends the mass range over which product ions can be collected.