AMAZING SEQUENCE ARRANGEMENT AT 5' ENDS OF ADENOVIRUS-2 MESSENGER-RNA
AMAZING SEQUENCE ARRANGEMENT AT 5' ENDS OF ADENOVIRUS-2 MESSENGER-RNA
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DOI：
10.1016/0092-8674(77)90180-5
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
ROBERTS, RJ
中科院分区：
文献类型：
--
作者：
CHOW, LT;GELINAS, RE;ROBERTS, RJ
在感染后期分离的几个腺病毒2型(AD2)基因的5‘末端序列与AD2基因组内的序列互补，而AD2基因组中的序列远离转录每个mRNA的主要编码序列的DNA。这是通过在AD2 DNA和来自感染的[人宫颈癌HeLa和口腔癌KB]细胞的未分离的多聚体RNA之间形成RNA置换环(R环)来观察到的。R环的5‘末端序列位于36-92位之间，与[Bal I，BamH-I，Hind III，Sal I和Xma I]限制性内切酶片段的单链DNA形成复杂的二级杂交，其中含有AD2基因组第36位左侧的序列。EM显示，编码在R链上MAP位置16.6、19.6和26.6的短序列在许多晚期mRNA的5‘端连接在一起，形成了150-200个核苷酸的前导序列。位于16.6位左侧的晚期mRNA显示了不同的第二点杂交模式。它包含4.9-6.0的序列，直接链接到9.6-10.9的序列。这些发现暗示了一种在哺乳动物细胞中生物合成AD2 mRNA的新机制。
The 5'' terminal sequences of several adenovirus 2 (Ad2) mRNA, isolated late in infection, are complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed. This was observed by forming RNA displacement loops (R loops) between Ad2 DNA and unfractionated polysomal RNA from infected [human cervical carcinoma HeLa and oral carcinoma KB] cells. The 5'' terminal sequences of mRNA in R loops, variously located between positions 36 and 92, form complex secondary hybrids with single-stranded DNA from [Bal I, BamH-I, Hind III, Sal I and Xma I] restriction endonuclease fragments containing sequences to the left of position 36 on the Ad2 genome. The structures visualized by EM show that short sequences coded at map positions 16.6, 19.6 and 26.6 on the R strand are joined to form a leader sequence of 150-200 nucleotides at the 5'' end of many late mRNA. A late mRNA which maps to the left of position 16.6 shows a different pattern of 2nd site hybridization. It contains sequences from 4.9-6.0 linked directly to those from 9.6-10.9. These findings imply a new mechanism for the biosynthesis of Ad2 mRNA in mammalian cells.