Techniques for advanced glycation end product measurements for diabetic bone disease: pitfalls and future directions.
Techniques for advanced glycation end product measurements for diabetic bone disease: pitfalls and future directions.
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DOI：
10.1097/med.0000000000000736
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发表时间：
2022-08-01
影响因子：
3.2
通讯作者：
Vashishth, Deepak
中科院分区：
文献类型：
--
作者：
Sroga, Grazyna E.;Stephen, Samuel J.;Wang, Bowen;Vashishth, Deepak
关键词：
Multiple biochemical and biophysical approaches have been broadly used for detection and quantitation of post-translational protein modifications associated with diabetic bone, yet these techniques present a variety of challenges. In this review, we discuss recent advancements and complementary roles of analytical (UPLC/UPLC-MS/MS and ELISA) and biophysical (Raman and FTIR) techniques used for characterization of glycation products, measured from bone matrix and serum, and provide recommendations regarding the selection of a technique for specific study of diabetic bone. Hyperglycemia and oxidative stress in diabetes contribute to the formation of a large subgroup of advanced glycation end products (AGEs) known as glycoxidation end products (AGOEs). AGEs/AGOEs have various adverse effects on bone health. Commonly, accumulation of AGEs/AGOEs leads to increased bone fragility. For example, recent studies show that carboxymethyllysine (CML) and pentosidine (PEN) are formed in bone at higher levels in certain diseases and metabolic conditions, in particular in diabetes and aging. Detection and quantitation of AGEs/AGOEs in rare and/or precious samples is feasible due to a number of technological advancements of the past decade. Recent technological advancements have led to a significant improvement of several key analytical biochemistry and biophysics techniques used for detection and characterization of AGEs/AGOEs in bone and serum. Their principles and applications to skeletal tissue studies as well as limitations are discussed in this review.
影响因子：
4.1
作者：
Mulani FA;Nandikol SS;Haldar S;Thulasiram HV
通讯作者：
Thulasiram HV