Functional characterization of podocan, a member of a new class in the small leucine-rich repeat protein family
Functional characterization of podocan, a member of a new class in the small leucine-rich repeat protein family
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DOI：
10.1016/s0014-5793(04)00250-9
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发表时间：
2004-04-09
期刊：
影响因子：
3.5
通讯作者：
Saruta, T
中科院分区：
文献类型：
--
作者：
Shimizu-Hirota, R;Sasamura, H;Saruta, T
细胞外基质的一个重要组成部分是一组非胶原蛋白，属于富含亮氨酸的小重复序列 (SLR) 蛋白家族。最近从肾脏中鉴定出一种新的 SLR 蛋白 Podocan，其结构特征不同于已知的 SLR 蛋白家族类别。在这项研究中，我们检查了培养细胞中表达的这种 SLR 蛋白的功能特征。在细胞内清楚地观察到 Podocan，并且在上清液中也可检测到。用各种糖酶处理表达的蛋白质表明，podocan 是一种含有 N-连接寡糖的糖蛋白，但不是经典的蛋白聚糖。此外，还发现 Podocan 可以结合 1 型胶原蛋白。转染 Podocan 的细胞显示细胞生长和迁移减少，同时 p21 表达增加。通过逆转录聚合酶链反应不仅在肾脏中检测到了 Podocan mRNA，而且在包括心脏和血管平滑肌细胞在内的其他组织中也检测到了 Podocan mRNA，这表明 Podocan 可能在心血管组织的生长调节中具有潜在作用。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
An important component of the extracellular matrix is the group of non-collagenous proteins belonging to the small leucine-rich repeat (SLR) protein family. A new SLR protein, podocan, with structural characteristics different from the known classes of the SLR protein family has been identified recently from the kidney. In this study, we examined the functional characteristics of this SLR protein expressed in cultured cells. Podocan was clearly observed intracellularly and was also detectable in the supernatant. Treatment of the expressed protein with various glycoenzymes suggested that podocan is a glycoprotein containing N-linked oligosaccharides but not a classical proteoglycan. Moreover, podocan was found to bind type 1 collagen. Cells transfected with podocan showed reductions in cell growth and migration, concomitant with increased p21 expression. Podocan mRNA was detected by reverse transcription polymerase chain reaction not only in the kidney, but also in other tissues including the heart and vascular smooth muscle cells, suggesting that podocan may have a potential role in growth regulation in cardiovascular tissues. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.