Decrease of CD4+CD25+ regulatory T cells and TGF-β at early immune reconstitution is associated to the onset and severity of graft-versus-host disease following allogeneic haematogenesis stem cell transplantation
Decrease of CD4+CD25+ regulatory T cells and TGF-β at early immune reconstitution is associated to the onset and severity of graft-versus-host disease following allogeneic haematogenesis stem cell transplantation
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DOI：
10.1016/j.leukres.2010.03.017
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发表时间：
2010-09-01
影响因子：
2.7
通讯作者：
Wang, Yiping
中科院分区：
文献类型：
--
作者：
Li, Qing;Zhai, Zhimin;Wang, Yiping
移植物抗宿主病（GVHD）是异基因造血干细胞移植（aHSCT）常见的危及生命的并发症。aHSCT后患者中CD 4（+）CD 25（+）调节性T细胞（T细胞）与急性和慢性GVHD（aGVHD和cGVHD）的发生和严重程度的相关性尚未得到充分研究。在这里，我们通过评估外周血中的CD 4（+）CD 25（高）和CD 4（+）CD 25（+）CD 127（低）以及血清TGF-β和TNF-α水平来检查CD 4（+）CD 25（+）Tregs的水平。aHSCT后早期免疫重建的56名患者。我们的数据显示，与健康对照组相比，II-IV级aGVHD和广泛cGVHD患者的TCRs频率显著降低。CD 4（+）CD 25（+）T细胞亚群水平降低与GVHD严重程度增加相关。非GVHD组CD 4（+）CD 25（+）T淋巴细胞水平显著高于健康对照组。随着GVHD严重程度的增加，还观察到TGF-β水平的显著降低和TNF-α水平的显著增加。本研究提示，在aHSCT后早期免疫重建时检测CD 4（+）CD 25（+）T细胞沿着血清TGF-β和TNF-α可能提示aGVHD和cGVHD的发生和严重程度。(C)2010爱思唯尔有限公司保留所有权利。
Graft-versus-host disease (GVHD) is a frequent and life threatening complication of allogeneic haematogenesis stem cell transplantation (aHSCT). The correlation of CD4(+)CD25(+) regulatory T cells (Tregs) in the patients after aHSCT to the occurrence and severity of acute and chronic GVHD (aGVHD and cGVHD) is not fully investigated. Here, we examined the levels of CD4(+)CD25(+) Tregs by assessment of CD4(+)CD25(high) and CD4(+)CD25(+)CD127(low) in peripheral blood, and the levels of serum TGF-beta and TNF-alpha in 56 patients at early immune reconstitution following aHSCT. Our data showed a significant reduction in the frequency of Tregs in patients with grades II-IV aGVHD and extensive cGVHD compared to healthy controls. Moreover, a decreased level of CD4(+)CD25(+) Tregs was correlated to increased severity of GVHD. The levels of CD4(+)CD25(+) Tregs in non-GVHD groups were however significantly higher than that in healthy controls. A significant decrease in the levels of TGF-beta and a significant increase the levels of TNF-alpha was also seen with increased severity of GVHD. This study suggested that measurement of CD4(+)CD25(+) Tregs along with serum TGF-beta and TNF-alpha at early immune reconstruction after aHSCT may indicate the onset and severity of both aGVHD and cGVHD. (C) 2010 Elsevier Ltd. All rights reserved.