Pancreatic Juice Exosomal MicroRNAs as Biomarkers for Detection of Pancreatic Ductal Adenocarcinoma
Pancreatic Juice Exosomal MicroRNAs as Biomarkers for Detection of Pancreatic Ductal Adenocarcinoma
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DOI：
10.1245/s10434-019-07269-z
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发表时间：
2019-07-01
影响因子：
3.7
通讯作者：
Nakamura, Masafumi
中科院分区：
文献类型：
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作者：
Nakamura, So;Sadakari, Yoshihiko;Nakamura, Masafumi
背景胰腺导管腺癌（PDAC）是一种致命的肿瘤，因为在早期发现的困难。最近的几项研究表明，外泌体可能具有作为新型生物标志物的潜力。本研究旨在从胰液中分离外泌体并研究外泌体microRNA（ex-miRs）是否可用作PDAC的生物标志物。采用内镜逆行胰管造影术采集PDAC和慢性胰腺炎（CP）患者的胰液。通过超离心法提取外来体。通过电子显微镜和使用抗-CD 63、-CD 81和-TSG 101抗体的蛋白质印迹法确认外泌体的存在。对PDAC和CP患者的ex-miR-21和ex-miR-155的相对水平进行定量和比较。共收集35份胰液样本（27份PDAC和8份CP）。PDAC患者中ex-miR-21和ex-miR-155的相对水平显著高于CP患者（p
Background. Pancreatic ductal adenocarcinoma (PDAC) is a lethal neoplasm because of difficulties in early detection. Several studies have recently suggested that exosomes may have potential as novel biomarkers. This study aimed to isolate exosomes from pancreatic juice and to investigate whether exosomal microRNAs (ex-miRs) could be used as biomarkers for PDAC.Methods. Pancreatic juice was collected from patients with PDAC and chronic pancreatitis (CP) by endoscopic retrograde pancreatography. Exosomes were extracted by ultracentrifugation. The presence of exosomes was confirmed by electron microscopy and Western blotting using anti-CD63, -CD81, and -TSG101 antibodies. Relative levels of ex-miR-21 and ex-miR-155 were quantified and compared between PDAC and CP patients.Results. A total of 35 pancreatic juice samples (27 PDAC and 8 CP) were collected. Relative levels of both ex-miR-21 and ex-miR-155 were significantly higher in PDAC patients compared with CP patients (p