Bovine serum albumin release from poly(alpha-hydroxy acid) microspheres: Effects of polymer molecular weight and surface properties
Bovine serum albumin release from poly(alpha-hydroxy acid) microspheres: Effects of polymer molecular weight and surface properties
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DOI：
10.1016/s0168-3659(96)01547-7
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发表时间：
1997-03-03
影响因子：
10.8
通讯作者：
Benoit, JP
中科院分区：
文献类型：
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作者：
Boury, F;Marchais, H;Benoit, JP
用两种市售的PLGA聚合物(乳酸/乙醇酸50：50，分子量分别为87和15 kDa)制备了含有模型蛋白牛血清白蛋白(BSA)的微球，该过程基于(油包水)乳化溶剂挥发/提取技术。考察了处方参数对牛血清白蛋白体外释放的影响。结果表明，包衣聚合物的包封率和蛋白质释放率均与包衣聚合物的相对分子质量密切相关。低分子量聚合物包封率的提高和几乎没有破裂效应的原因是蛋白质与壁状聚合物之间的离子和物理相互作用。此外，接触角测量表明微球表面没有蛋白质，并表明用最高相对分子质量的聚合物测量的破裂效应不能用蛋白质从表面解吸来解释，而是用蛋白质从微球表面附近的区域扩散来解释。
Two commercially available PLGA polymers (lactic acid/glycolic acid 50:50, with respective molecular weights of 87 and 15 kDa) were used to prepare microspheres containing a model protein, bovine serum albumin (BSA), following a process based on the (water-in-oil)-in-water emulsion solvent evaporation/extraction technique. The influence of the formulation parameters on the in vitro release profiles of BSA was assessed. The results showed that both the encapsulation efficiency and the protein release rate were closely dependent on the molecular weight of the coating polymer. The increase of the encapsulation efficiency, and the quasi-absence of burst effect with the low molecular weight polymer, are explained by ionic and physical interactions between the protein and the wall polymer. In addition, contact angle measurements revealed the absence of protein at the surface of the microspheres and showed that the burst effect, measured with the highest molecular weight polymer, could not be explained by desorption of the protein from the surface but rather by the diffusion of the protein from regions bordering the microsphere surface.