Ezrin in osteosarcoma: Comparison between conventional high-grade and central low-grade osteosarcoma
Ezrin in osteosarcoma: Comparison between conventional high-grade and central low-grade osteosarcoma
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DOI：
10.1016/j.prp.2006.01.015
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Park, Yong-Koo
中科院分区：
文献类型：
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作者：
Park, Hye-Rim;Jung, Woon Won;Park, Yong-Koo
Ezrin是一种细胞骨架连接蛋白，积极参与调节癌细胞的生长和转移能力。最近，有研究表明ezrin高表达水平与儿童骨肉瘤患者预后不良之间存在显著相关性。比较ezrin在常规高级别骨肉瘤和中央性低级别骨肉瘤病变中的表达，探讨ezrin过表达在骨肉瘤转移中的作用。我们用免疫组化方法比较了32例高级别骨肉瘤和21例低级别骨肉瘤中ezrin蛋白的表达水平。采用Western blotting检测Ezrin蛋白在3种不同人骨肉瘤细胞系中的表达水平。通过实时定量PCR检测ezrin mRNA在骨肉瘤细胞系和对照成纤维细胞中的表达水平。43.7%的高级别骨肉瘤标本存在Ezrin免疫反应性。所有低级别骨肉瘤ezrin均为阴性。Western blotting检测ezrin在3种骨肉瘤细胞系中的表达。与对照细胞相比，所测试的骨肉瘤细胞系显示ezrin mRNA明显扩增。综上所述，ezrin似乎在肿瘤的进展中发挥作用，例如骨肉瘤的转移。然而，在骨肉瘤患者的临床决策中考虑ezrin之前，还需要进一步的数据。(c) 2006 Elsevier GmbH版权所有。
Ezrin is a cytoskeleton linker protein that is actively involved in the regulation of growth and metastatic capacity of cancer cells. Recently, it has been demonstrated that a significant correlation exists between high ezrin expression levels and the poor outcome of pediatric osteosarcoma patients. The expression of ezrin was compared in conventional high-grade and central low-grade ostcosarcoma lesions to investigate the role of ezrin overexpression in the metastasis of osteosarcoma.We compared the expression levels of the ezrin protein in 32 cases of high-grade osteosarcomas and 21 cases of low-grade osteosarcomas using immunohistochemistry. Ezrin protein expression levels were examined in three different human osteosarcoma cell lines by Western blotting. In addition, the mRNA expression levels of ezrin in these osteosarcoma cell lines and control fibroblasts were evaluated by real-time quantitative PCR.Ezrin immunoreactivity was present in 43.7% of high-grade osteosarcoma specimens. All low-grade osteosarcomas were negative for ezrin. The expression of ezrin was detected by Western blotting in all three osteosarcoma cell lines. The tested osteosarcoma cell lines showed marked amplification of ezrin mRNA compared to control cells. Taken together, ezrin appears to play a role in the progression of tumors, such as the metastasis of osteosarcoma. However, further data are needed before ezrin can be considered in clinical decision-making about osteosarcoma patients. (c) 2006 Elsevier GmbH. All rights reserved.