Evaluation of dynamic change of serum miR-21 and miR-24 in pre- and post-operative lung carcinoma patients
Evaluation of dynamic change of serum miR-21 and miR-24 in pre- and post-operative lung carcinoma patients
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DOI：
10.1007/s12032-012-0303-z
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发表时间：
2012-12-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Yong-Kui
中科院分区：
文献类型：
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作者：
Le, Han-Bo;Zhu, Wang-Yu;Zhang, Yong-Kui
虽然在癌症患者血清中经常检测到循环microRNAs(MiRNAs)，但对肺癌患者手术前后血清中miRNAs表达的动态变化尚缺乏分析。因此，我们采用定量逆转录聚合酶链式反应(qRT-PCR)检测了82例肺癌患者手术前和术后10天配对患者以及50名正常志愿者血清中四种miR-21、miR-205、miR-30d和miR-24的表达。我们发现，与正常志愿者相比，miR-21、miR-205、miR-30d和miR-24在肺癌患者血清中的表达增加，在早期肺癌患者的血清中也是如此。血清miR-21、miR-205、miR-30d和miR-24水平的ROC曲线下面积(AUC)显著高于癌胚抗原(CEA)水平(P<0.05)，而联合检测miRNA和CEA的AUC与单独检测miRNAs的AUC差异无统计学意义(P>；0.05)。术后血清中miR-21和miR-24的表达水平较术前显著降低(P分别为0.0004和0.0001)。此外，术前血清miR-21和miR-30d的高表达与肺癌患者的总生存期缩短独立相关(LOG-RANK检验：P=0.0498，0.0019)。综上所述，我们的结果提示miR-21、miR-205、miR-30d和miR-24可能成为诊断肺癌的新的非侵袭性生物标志物。此外，术后血清miR-21和miR-24水平低于术前，提示它们有可能作为术后复发的生物标志物。
Although circulating microRNAs (miRNAs) were frequently detected in sera of cancer patients, there is still a lack of analysis of the dynamic changes of miRNAs expression in sera of pre- and post-operative lung carcinoma patients. Thus, we conducted quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to examine the expression of four miRNAs (miR-21, miR-205, miR-30d, and miR-24) in the sera of a set of 82 pre-operative lung carcinoma patients and paired 10 days post-operative patients, as well as in 50 normal volunteers. We showed that, compared to that in normal volunteers, the expression of miR-21, miR-205, miR-30d, and miR-24 was increased in lung cancer sera samples, as well as in sera of early stage lung cancer patients according to their clinical-pathological characteristics. The area under roc curves (AUCs) for levels of miR-21, miR-205, miR-30d, and miR-24 in sera were significantly higher than those for Carcinoma embryonic antigen (CEA) (P < 0.05), whereas the AUC for combination of serum levels of miRNA with serum CEA showed no significant difference from that for serum levels of miRNAs only (P > 0.05). The expression levels of miR-21 and miR-24 were significantly decreased in post-operative sera compared with levels in paired pre-operative sera (P = 0.0004 and < 0.0001, respectively). In addition, high expressions of miR-21 and miR-30d in pre-operative sera were independently correlated with shorter overall survival in lung cancer patients (log-rank test: P = 0.0498, 0.0019). In summary, our results suggest that miR-21, miR-205, miR-30d, and miR-24 may serve as potential novel non-invasive biomarkers for diagnosis of lung cancer. In addition, miR-21 and miR-24 serum levels were lower in post-operative samples than those in pre-operative samples, suggesting they can potentially be used as biomarkers for disease recurrence after surgery operation.