Utility of diagnostic tests used in diagnosis of infection in dogs experimentally inoculated with a North American isolate of Leishmania infantum infantum.
Utility of diagnostic tests used in diagnosis of infection in dogs experimentally inoculated with a North American isolate of Leishmania infantum infantum.
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诊断测试用于诊断实验性接种北美婴儿利什曼原虫分离株的狗的感染。
DOI：
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发表时间：
2005
影响因子：
2.6
通讯作者：
D. Lindsay
中科院分区：
文献类型：
--
作者：
A. Rosypal;G. Troy;R. Duncan;A. Zajac;D. Lindsay
用1 × 10(7)（低剂量，LD）或2 × 10(8)（高剂量，HD）从自然感染的弗吉尼亚猎狐犬中分离的北美利什曼原虫（LIVT-1株）原鞭毛虫感染了8只雌性比格犬。阴性对照为2只雌性比格犬，阳性对照为2只慢性感染（10 ~ 30岁）恰加什婴儿利什曼原虫的雄性比格犬。每6-8周收集一次骨髓（BM）和淋巴结（LN）标本，进行细胞学评估、寄生虫培养和聚合酶链反应（PCR）。每月采集血清样本，通过间接荧光抗体试验（IFAT）和诊断性rK39抗原测定血清学反应。在研究过程中，阳性对照犬的BM和LN吸痰培养和细胞学评估始终呈阳性。阴性对照犬BM和LN培养及吸出液细胞学评估均为阴性。2只实验感染犬BM抽吸液细胞学检查均发现无刚体。所有犬中，22个样本LN抽吸培养呈阳性，12个样本BM培养呈阳性。在研究过程中，实验感染犬的IFA滴度从0到1:400不等。在感染后约8周，阳性对照犬和HD犬的重组K39免疫分析测试始终呈阳性。与LD犬相比，HD犬的BM PCR产物鉴定更加一致。Kappa统计表明，在实验感染的狗中，与IFAT或rK39免疫测定结果相比，PCR与培养和细胞学的相关性更好。
Eight female beagles were infected with 1 x 10(7) (low dose, LD) or 2 x 10(8) (high dose, HD) promastigotes of a North American isolate of Leishmania infantum infantum (LIVT-1 strain) isolated from naturally infected Virginia Foxhounds. Two female beagles served as negative controls and 2 male beagles chronically infected (> 3 years) with Leishmania infantum chagasi were positive controls. Bone marrow (BM) and lymph node (LN) aspirates were collected every 6-8 weeks for cytologic evaluation, parasite culture, and polymerase chain reaction (PCR). Serum samples were collected monthly for determination of serologic responses by indirect fluorescent antibody test (IFAT) and diagnostic rK39 antigen. Cultures of BM and LN aspirates and cytology evaluation were consistently positive in positive control dogs during the course of study. Negative control dogs were negative on BM and LN cultures and on cytologic evaluation of aspirates. Amastigotes were present on cytological examination of BM aspirates in 2 experimentally infected dogs. Cultures of LN aspirates were positive on 22 samples, whereas BM cultures were positive on 12 samples for all dogs. IFA titers ranged from 0 to 1 :400 in experimentally infected dogs during the course of the study. Recombinant K39 immunoassay tests were consistently positive in positive control dogs and in the HD dogs by approximately 8 weeks after infection. BM PCR products were identified more consistently in the HD dogs compared with the LD dogs. Kappa statistics indicated PCR correlated better with cultures and cytology than did IFAT or the rK39 immunoassay results in the experimentally infected dogs.