EARLY EVENTS IN INITIATION OF ALTERNATIVE COMPLEMENT PATHWAY ACTIVATION BY THE CAPSULE OF CRYPTOCOCCUS-NEOFORMANS
EARLY EVENTS IN INITIATION OF ALTERNATIVE COMPLEMENT PATHWAY ACTIVATION BY THE CAPSULE OF CRYPTOCOCCUS-NEOFORMANS
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DOI：
10.1128/iai.59.9.3101-3110.1991
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发表时间：
1991-09-01
影响因子：
3.1
通讯作者：
MURPHY, JW
中科院分区：
文献类型：
--
作者：
KOZEL, TR;WILSON, MA;MURPHY, JW
新型隐球菌囊是补体替代途径的强力激活剂。本研究考察了隐球菌胶囊影响新生隐球菌补体激活的启动和早期事件的方式。这些研究考察了经典途径和替代途径对C3片段在被包裹的隐球菌、非被包裹的隐球菌和酶酶体上沉积的动力学和早期位点的影响。结果表明，未包封的隐球菌和酶酶san在启动补体激活的方式上在质量和数量上相似。两者都利用经典和替代途径。通过经典途径的起始在分布于整个细胞表面的位点上突然同时发生。酶酵素和非包膜隐球菌启动替代途径的特点是在可观数量的C3积聚在细胞上之前延迟6至8分钟。酶酵素和非包膜隐球菌的替代途径起始发生在有限数量的局灶起始位点，这些位点随着替代途径扩增而扩大，覆盖细胞表面。隐球菌囊的存在阻断了通常发生在隐球菌细胞壁的经典途径启动，并产生了仅依赖于替代途径的启动。隐球菌包膜启动替代途径的特点是C3积累滞后，出现有限数量的局灶起始位点，这与替代途径被酶酵素和非包膜隐球菌激活时所观察到的情况相似。
The capsule of Cryptococcus neoformans is a powerful activator of the alternative complement pathway. This study examined the manner in which the cryptococcal capsule influences initiation of and early events in complement activation by C. neoformans. These studies examined the effects of the classical and alternative pathways on the kinetics and early sites for deposition of C3 fragments on encapsulated cryptococci, nonencapsulated cryptococci, and zymosan. The results showed that nonencapsulated cryptococci and zymosan are qualitatively and quantitatively similar in the manner in which they initiate complement activation. Both utilize the classical and alternative pathways. Initiation via the classical pathway occurs suddenly and simultaneously at sites distributed over the entire cell surface. Initiation of the alternative pathway by zymosan and nonencapsulated cryptococci is characterized by a lag of 6 to 8 min before appreciable amounts of C3 accumulate on the cells. Alternative pathway initiation by zymosan and nonencapsulated cryptococci occurs at a limited number of focal initiation sites that expand with alternative pathway amplification to cover the cell surface. Presence of the cryptococcal capsule blocks classical pathway initiation, which would normally occur at the cryptococcal cell wall, and produces an initiation that is dependent solely on the alternative pathway. Initiation of the alternative pathway by the cryptococcal capsule is characterized by a lag in C3 accumulation and the appearance of a limited number of focal initiation sites which resemble those observed when the alternative pathway is activated by zymosan and nonencapsulated cryptococci.