Genomic expression responses toward bisphenol-A toxicity in Daphnia magna in terms of reproductive activity
Genomic expression responses toward bisphenol-A toxicity in Daphnia magna in terms of reproductive activity
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DOI：
10.1007/s13273-013-0019-y
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发表时间：
2013-06-30
影响因子：
1.7
通讯作者：
Seo, Young Rok
中科院分区：
文献类型：
--
作者：
Jeong, Seok Won;Lee, Sang Min;Seo, Young Rok
双酚A（BPA）被归类为内分泌干扰物，可干扰内分泌系统，对人类和野生动物的发育、生殖、神经和免疫产生不良影响。为了研究双酚A对水生生态系统的生殖和遗传毒性效应，以大型蚤（Daphnia magna）为实验材料，研究了双酚A对水生生态系统的生殖毒性效应。Magna）作为试验动物模型。D. magna是世界上生态毒理学测试中使用最广泛的水生物种之一。结果，与对照组相比，在暴露于10 mg/L BPA的21天内，基于后代生产数量的生殖活动显著降低。随后使用DNA微阵列分析进行了响应BPA暴露的基因表达谱。我们的数据表明，许多假定的基因的核苷酸序列同源性的动物模型，包括表皮蛋白，卵黄蛋白原，蛋白酶，核糖体蛋白家族的表达水平发生了变化。这些在其他动物系统中被严格报道的基因可以被认为是响应BPA暴露的新生物标志物。
Bisphenol-A (BPA) classified as an endocrine disruptor that can interfere endocrine system, leading to adverse developmental, reproductive, neurological, and immunological effects in both humans and wildlife. To study the reproductive and geno-toxicological effects of BPA in aquatic ecosystem, we employed Daphnia magna (D. magna) as a test animal model. D. magna in one of the most widely used water species employed in ecotoxicology testing all over the world. As a result, the reproductive activity based on the number of offspring production is significantly reduced toward 21 day exposure to 10 mg/L of BPA, compared with control. Gene expression profiles in response to BPA exposure using DNA microarray analysis was subsequently carried out. Our data showed that expression levels of numerous putative genes with nucleotide sequences homology to animal models including cuticular protein, vitellogenin, protease, ribosomal protein family were altered. These genes which are critically reported in other animal systems could be considered as novel biomarkers in response to BPA exposure.