ACTIVATION OF HUMAN LYMPHOCYTES-T .2. INVOLVEMENT OF THE T3 ANTIGEN IN POLYCLONAL T-CELL ACTIVATION BY MITOGENIC LECTINS AND OXIDATION
ACTIVATION OF HUMAN LYMPHOCYTES-T .2. INVOLVEMENT OF THE T3 ANTIGEN IN POLYCLONAL T-CELL ACTIVATION BY MITOGENIC LECTINS AND OXIDATION
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DOI：
10.1002/eji.1830140815
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
FLEISCHER, B
中科院分区：
文献类型：
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作者：
FLEISCHER, B
研究了T细胞表面抗原T3和T4单克隆抗体对辅助细胞依赖性丝裂原诱导的人抗原特异性T4+ T淋巴细胞克隆和纯化外周血T细胞增殖的影响。为了避免OKT3抗体的Fc受体依赖性有丝分裂作用，单核细胞被Epstein-Barr病毒转化的B淋巴母细胞样细胞取代为辅助细胞。单克隆抗体OKT3而非OKT4抑制T淋巴细胞克隆和纯化T细胞对有丝分裂凝集素和氧化的反应。这种抑制不是由于单克隆抗体的非特异性作用，因为它只影响初始触发而不影响活化T细胞的增殖，并且可以通过高浓度的凝集素来克服，这表明OKT3抗体和凝集素之间存在竞争。在该系统中具有抑制作用的相同浓度的OKT3抗体在携带Fc受体的单核细胞存在下具有丝分裂性。表面调节T3而非T4抗原导致对调节后直接给予的有丝分裂原脉冲无反应。显然，抗原特异性和丝裂原诱导的T细胞触发是由于与T淋巴细胞相同受体的相互作用。
The effect of monoclonal antibodies against the T cell surface antigens T3 and T4 on accessory cell-dependent mitogen-induced proliferation of human antigen-specific T4+ T lymphocyte clones and purified peripheral blood T cells was studied. To avoid the Fc receptor-dependent mitogenic effects of the OKT3 antibodies, monocytes were replaced by Epstein-Barr virus-transformed B lymphoblastoid cells as accessory cells. Monoclonal antibody OKT3 but not OKT4 inhibited the response of both T lymphocyte clones and purified T cells to mitogenic lectins and oxidation. The inhibition was not due to nonspecific effects of the monoclonal antibodies since it affected only the initial triggering but not the proliferation of activated T cells and it could be overcome by higher concentrations of lectin indicating a competition between OKT3 antibody and lectin. OKT3 antibody at the same concentration that was inhibitory in this system was mitogenic in the presence of Fc receptor-bearing monocytes. Surface modulation of T3 but not of T4 antigens led to unresponsiveness to a mitogen pulse given directly after modulation. Evidently, antigen-specific and mitogen-induced T cell triggering are due to interaction with the same receptors of the T lymphocyte.