REGIONAL VARIATION IN THE EXPRESSION OF BULLOUS PEMPHIGOID ANTIGEN AND LOCATION OF LESIONS IN BULLOUS PEMPHIGOID
REGIONAL VARIATION IN THE EXPRESSION OF BULLOUS PEMPHIGOID ANTIGEN AND LOCATION OF LESIONS IN BULLOUS PEMPHIGOID
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DOI：
10.1111/1523-1747.ep12260627
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发表时间：
1984-01-01
影响因子：
6.5
通讯作者：
BYSTRYN, JC
中科院分区：
文献类型：
--
作者：
GOLDBERG, DJ;SABOLINSKI, M;BYSTRYN, JC
大疱性类天疱疮 (BP) 抗原在身体不同部位的表达是通过间接免疫荧光在某个部位出现阳性反应的 BP 血清的最高稀释度来估计的。三种具有高滴度 (640-1280) BP 抗体的抗猴和豚鼠食管血清与 46 个正常人皮肤样本发生反应。不同部位皮肤中BP抗原的表达存在显着差异。 BP抗原的最大表达是在从手臂、腿和大腿的屈肌表面获得的皮肤中。来自这些地点的所有样本均与高稀释度（1:640-1:2560）的 BP 抗体发生反应。 BP抗原在头皮、面部和手臂伸肌表面的皮肤标本中表达最少。来自这些位置的 17% 的样本中没有显示出 BP 抗原，其余样本仅对低稀释度（1:40-1:160）的 BP 抗体有反应。来自躯干、膝盖和大腿伸肌的皮肤含有中等量的 BP 抗原。所有 3 种 BP 血清均出现类似结果。在同一个体的不同部位获得的多个皮肤样本中观察到类似的反应。值得注意的是，BP 抗原的表达最多的是常见于该疾病的区域（弯曲表面），而表达最少的是很少涉及的区域（头皮、面部、伸臂）。 BP抗原的表达与大疱性类天疱疮皮损位置之间的明显相关性表明，BP抗原表达可能在该病皮损的分布中发挥作用。
The expression of bullous pemphigoid (BP) antigen in different locations on the body was estimated from the highest dilution of BP sera giving a positive reaction at a site by indirect immunofluorescence. Three sera with high titers (640-1280) of BP antibodies against monkey and guinea pig esophagus were reacted with 46 specimens of normal human skin. There was marked variation in the expression of BP antigen in skin obtained from different sites. The greatest expression of BP antigen was in skin obtained from the flexor surfaces of the arm, leg and thigh. All specimens from these sites reacted with high dilutions (1:640-1:2560) of BP antibodies. BP antigen was expressed least in skin specimens from scalp, face and extensor surface of the arm. No BP antigen could be demonstrated in 17% of specimens from these locations and the remainder reacted only with low dilutions (1:40-1:160) of BP antibodies Skin from trunk, knee and extensor thigh had intermediate amounts of BP antigen. Similar results were seen with all 3 BP sera. Similar reactions were observed in multiple skin specimens obtained from different sites in the same individual. Of note is that the greatest expression of BP antigen was in areas commonly involved in this disease (flexural surfaces), whereas the least was seen in areas (scalp, face, extensor arm) which are rarely involved. The apparent correlation between the expression of BP antigen and the location of lesions in bullous pemphigoid, suggests that BP antigen expression may play a role in the distribution of skin lesions in this disease.