Clinical Significance of MCM-2 and MCM-5 Expression in Colon Cancer: Association with Clinicopathological Parameters and Tumor Proliferative Capacity
Clinical Significance of MCM-2 and MCM-5 Expression in Colon Cancer: Association with Clinicopathological Parameters and Tumor Proliferative Capacity
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DOI：
10.1007/s10620-008-0305-z
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发表时间：
2009-02-01
影响因子：
3.1
通讯作者：
Theocharis, Stamatios
中科院分区：
文献类型：
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作者：
Giaginis, Constantinos;Georgiadou, Maria;Theocharis, Stamatios
微小染色体维持（MCM）蛋白是DNA复制的重要组成部分，与细胞增殖有关，并作为癌症筛查、监测和预后的有用标志物。我们的目的是研究MCM-2和MCM-5蛋白表达在结肠癌中的临床意义，并评估其与各种临床病理特征和肿瘤增殖能力的关系。MCM-2和MCM-5的免疫组织化学表达的石蜡包埋的恶性组织切片从96例结肠癌患者。MCM-2和MCM-5表达与不同的临床病理特征、增殖能力（Ki-67标记指数）和p53细胞周期调节因子表达相关。MCM-2和Ki-67的表达与肿瘤的组织学分级显著相关（P = 0.003），存在结节转移（N）（P = 0.003和P = 0.030），腺瘤上的恶性（分别为P = 0.029和P = 0.024）和血管侵犯（分别为P = 0.010和P = 0.011）。MCM-2表达与Dukes分期有关（P = 0.005）。MCM-2、MCM-5蛋白表达与Ki-67蛋白表达之间存在显著正相关（r = 0.963，P值< 0.001; r = 0.738，P值< 0.001），MCM-2与MCM-5蛋白表达之间存在显著正相关（r = 0.745，P值< 0.001）。MCM-2、MCM-5蛋白表达与p53蛋白表达呈显著正相关，但均低于Ki-67蛋白表达。MCM-5蛋白表达与临床病理特征无明显相关性。目前的数据表明，MCM-2蛋白表达与患者管理的重要临床病理特征显著相关，与结肠癌中的细胞增殖状态相关。
Minichromosome maintenance (MCM) proteins are essential components of DNA replication, being related to cell proliferation, and serve as useful markers for cancer screening, surveillance, and prognosis. Our aim was to examine the clinical significance of MCM-2 and MCM-5 protein expression in colon cancer and to evaluate the association with various clinicopathological characteristics and tumor proliferative capacity. Immunohistochemical expression of MCM-2 and MCM-5 was performed on paraffin-embedded malignant tissue sections obtained from 96 patients with colon cancer. MCM-2 and MCM-5 expression was correlated with different clinicopathological characteristics, proliferative capacity (Ki-67 labeling index), and p53 cell-cycle regulator expression. MCM-2 and Ki-67 expression was significantly associated with the tumors' histological grade (P = 0.003), existence of nodular metastases (N) (P = 0.003 and P = 0.030, respectively), malignancy on adenoma (P = 0.029 and P = 0.024, respectively), and vascular invasion (P = 0.010 and P = 0.011, respectively). MCM-2 expression was additionally associated with Dukes' stage (P = 0.005). Significant positive relationships were found between the expression of MCM-2 or MCM-5 proteins and that of Ki-67 protein (r = 0.963, P-value < 0.001, and r = 0.738, P-value < 0.001, respectively), as well as between MCM-2 and MCM-5 proteins (r = 0.745, P-value < 0.001). Significant positive relationships were also observed between the expression of MCM-2 or MCM-5 proteins and that of p53 protein; however, they were consistently lower than the corresponding with Ki-67 protein. No significant association was observed between MCM-5 protein expression and the clinicopathological characteristics examined. The current data suggest that MCM-2 protein expression is significantly associated with important clinicopathological characteristics for patients' management, being correlated with the cell proliferation state in colon cancer.