Increased Production of Vascular Endothelial Growth Factor-D and Lymphangiogenesis in Acute Kawasaki Disease
Increased Production of Vascular Endothelial Growth Factor-D and Lymphangiogenesis in Acute Kawasaki Disease
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DOI：
10.1253/circj.cj-10-0897
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Kohno, Yoichi
中科院分区：
文献类型：
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作者：
Ebata, Ryota;Abe, Jun;Kohno, Yoichi
背景：川崎病（KD）的特点是全身性血管炎伴组织水肿。在炎症愈合过程中，淋巴管生成对于减少组织水肿至关重要。血管内皮生长因子-D (VEGF-D) 是急性 KD 愈合过程中诱导淋巴管生成的一种潜在候选药物。 方法和结果：使用酶联免疫吸附测定法研究了急性 KD 患者 (n=47) 血清 VEGF-D 水平的连续变化。通过免疫组织化学方法评估死于 KD 的患者 (n=6) 心脏组织中的淋巴管横截面积和 VEGF-D 蛋白表达。使用定量实时聚合酶链反应评估培养成纤维细胞中 VEGF-D 信使 RNA (mRNA) 表达的调节。急性 KD 患者静脉注射免疫球蛋白治疗后血清 VEGF-D 水平升高（P
Background: Kawasaki disease (KD) is characterized by systemic vasculitis with tissue edema. During the healing process of inflammation, lymphangiogenesis is essential for reducing tissue edema. One potential responsible candidate for the induction of lymphangiogenesis in the healing process of acute KD is vascular endothelial growth factor-D (VEGF-D).Methods and Results: Sequential changes in serum VEGF-D levels in patients with acute KD (n=47) using an enzyme-linked immunosorbent assay were investigated. Cross-sectional areas of lymphatic vessels and VEGF-D protein expression were evaluated immunohistochemically in cardiac tissues of patients (n=6) who died of KD. Regulation of VEGF-D messenger RNA (mRNA) expression in cultured fibroblasts was assessed using quantitative real-time polymerase chain reaction. Serum VEGF-D levels increased after intravenous immunoglobulin therapy in patients with acute KD (P