Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets
Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets
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DOI：
10.7554/elife.44031
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发表时间：
2019-05-01
期刊：
影响因子：
7.7
通讯作者：
Nigrovic, Peter A.
中科院分区：
文献类型：
--
作者：
Cunin, Pierre;Bouslama, Rim;Nigrovic, Peter A.
骨髓巨核细胞吞噬中性粒细胞的现象称为empipolesis。在这里，我们表明，empipolesis是一个动态的过程中积极介导的两个谱系，部分通过β 2-整合素/ICAM-1/ezrin途径。系留的中性粒细胞在进入巨核细胞胞质之前进入膜结合囊泡。胞浆内中性粒细胞与分界膜系统形成膜接触，从而将膜转移到巨核细胞和子血小板。这种现象发生在体内其他未经处理的小鼠骨髓中，导致循环血小板携带来自非巨核细胞造血供体的膜。通过巨核细胞的转运可以在几分钟内完成，之后中性粒细胞完整地排出。在与血小板过度产生相关的鼠炎症模型中，帝国主义被放大，有助于体外和体内血小板的产生。这些发现将empipolesis确定为一种新的细胞间相互作用，其使中性粒细胞和可能通过巨核细胞细胞质的其他细胞能够调节血小板的产生和膜含量。
Bone marrow megakaryocytes engulf neutrophils in a phenomenon termed emperipolesis. We show here that emperipolesis is a dynamic process mediated actively by both lineages, in part through the beta 2-integrin/ICAM-1/ezrin pathway. Tethered neutrophils enter in membrane-bound vesicles before penetrating into the megakaryocyte cytoplasm. Intracytoplasmic neutrophils develop membrane contiguity with the demarcation membrane system, thereby transferring membrane to the megakaryocyte and to daughter platelets. This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. Transit through megakaryocytes can be completed as rapidly as minutes, after which neutrophils egress intact. Emperipolesis is amplified in models of murine inflammation associated with platelet overproduction, contributing to platelet production in vitro and in vivo. These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets.