Naturally arising point mutations in non-essential domains of equine infectious anemia virus Rev alter Rev-dependent nuclear-export activity.
Naturally arising point mutations in non-essential domains of equine infectious anemia virus Rev alter Rev-dependent nuclear-export activity.
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马传染性贫血病毒Rev非必需结构域中自然产生的点突变改变了Rev依赖性核输出活性。
DOI：
10.1099/vir.0.83195-0
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Carpenter,Susan
中科院分区：
文献类型：
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作者：
Sparks,WendyO;Dorman,KarinS;Liu,Sijun;Carpenter,Susan
马传染性贫血病毒 (EIAV) 在体内表现出很高的遗传变异率，并导致受感染的马产生临床变异疾病。EIAV 体内群体的特征是存在不同的 Rev 遗传亚群，这些亚群的表型不同，并且优势波动，其方式与疾病的每个临床阶段一致。这项研究检查了体内出现并改变表型的特定突变。 Rev 蛋白被发现高度保守，在样本群体中仅观察到 10 aa 突变，频率大于 10 %。其中九个突变能够显着改变 Rev 活性，无论是在创始人变体的背景下作为单个突变，还是在累积固定突变的背景下。结果表明，Rev 基本功能域之外的有限遗传变异可以改变表型，并可能赋予体内选择性优势。
Equine infectious anemia virus (EIAV) exhibits a high rate of genetic variationin vivo, and results in a clinically variable disease in infected horses.In vivopopulations of EIAV have been characterized by the presence of distinct, genetic subpopulations of Rev that differ in phenotype and fluctuate in dominance in a manner coincident with each clinical stage of disease. This study examined the specific mutations that arosein vivoand altered the phenotype. The Rev protein was found to be highly conserved, and only 10 aa mutations were observed at a frequency greater than 10 % in the sample population. Nine of these mutations were capable of significantly altering Rev activity, either as single mutations in the context of the founder variant, or in the context of cumulatively fixed mutations. The results indicated that limited genetic variation outside the essential functional domains of Rev can alter the phenotype and may confer a selective advantagein vivo.