Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria

Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria
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哈维氏弧菌和费氏弧菌荧光素酶在丝状蓝藻中的表达

DOI:
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发表时间:
1986
影响因子:
3.2
通讯作者:
J. Elhai
J. Elhai
中科院分区:
生物学3区
文献类型:
--
作者:
G. Schmetterer;C. Wolk;J. Elhai

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利用穿梭载体将哈维弧菌和费氏弧菌的lux基因转移到水藻中(蓝藻中荧光素酶的表达水平高达7000量子细胞-1 s-1),使这些基因很好地用作启动子探针,用于某些细胞在丝中分化为异囊。
Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.
蓝藻鱼腥藻 71​​20 中编码核酮糖-1,5-二磷酸羧化酶小亚基和大亚基的基因的共转录。
DOI: 10.1073/pnas.81.19.5961
发表时间: 1984
影响因子: 11.1
作者:
Nierzwicki-Bauer,SA;Curtis,SE;Haselkorn,R
通讯作者: Haselkorn,R
DOI: 10.1021/bi00311a014
发表时间: 1984-01-01
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
BALDWIN, TO;BERENDS, T;ZIEGLER, MM
通讯作者: ZIEGLER, MM