Antagonism of Xenorhabdus and Photorhabdus to Botrytis cinerea
Antagonism of Xenorhabdus and Photorhabdus to Botrytis cinerea
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发表时间：
2009
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通讯作者：
Cheng Shu-long
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作者：
Cheng Shu-long
测定了牛链杆菌HJ4菌株、牛疫杆菌HF22菌株、发光菌F30-4菌株和发光菌S21菌株对灰霉病菌的拮抗作用。结果表明，所有菌株的培养上清液对灰霉病菌都有不同程度的拮抗作用。10倍菌液对菌丝生长的5倍稀释度对菌丝生长的抑制率可达70%~85%，其中以发光菌HF22菌株的抑制率最高。此外，HF22菌株对菌丝生长的抑制作用最强细菌培养上清液对孢子萌发也有抑制作用，以HJ4菌株效果最好，5倍稀释液对孢子萌发的抑制率为34.98%，10倍稀释液对菌丝生长的抑制率分别为74.19%和18.92%，说明抑制菌丝生长的活性分子主要在细胞内。培养上清液在紫外光照射0.5~1h对其活力无明显影响，但在紫外光照射0.5h后，细胞活力显著降低。70℃培养10min后，细胞活力显著下降。
The antagonism of Xenorhabdus bovienii HJ4 strain,X.bovienii HF22 strain,Photorhabdus luminescens F30-4 strain and Photorhabdus sp.S21 strain to Botrytis cinerea were assayed.The results showed that the culture supernatant from all strains had antagonism to Botrytis cinerea to some extent.The inhibition of 5X dilution of bacterium culture to mycelium growth could reach as high as 70%-85%.50% inhibition was detected when treated with 10X bacterium culture.The HF22 strain gave the highest inhibition results.In addition,the supernatant of bacterium culture also inhibited the spore germination.The best result was obtained by HJ4 strain and its inhibition to spore germination was 34.98% with 5X dilution.The 10X dilution of HF22 strain cells and supernatant inhibited the mycelium growth to 74.19% and 18.92% respectively.It proved that the active molecule for inhibiting the mycelium growth was mainly in the cell.After being treated with different temperature or the UV light,the activity of the culture supernatant of HF22 strain was not affected by the higher temperature,but the activity of its cells was significantly decreased after incubated at 70℃ for 10 min.Exposing the supernatant to the UV light for 0.5-1 h did not significantly affect its activity,but the activity of the cells was significantly decreased even after exposure to the UV light for 0.5 h.