ANDROGEN METABOLISM AND INHIBITION OF INTERLEUKIN-1 SYNTHESIS IN PRIMARY CULTURED HUMAN SYNOVIAL MACROPHAGES
ANDROGEN METABOLISM AND INHIBITION OF INTERLEUKIN-1 SYNTHESIS IN PRIMARY CULTURED HUMAN SYNOVIAL MACROPHAGES
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DOI：
10.1155/s096293519500024x
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发表时间：
1995-03-01
影响因子：
4.6
通讯作者：
CASTAGNETTA, L
中科院分区：
文献类型：
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作者：
CUTOLO, M;ACCARDO, S;CASTAGNETTA, L
在人类正常和类风湿滑膜组织中，雄激素受体存在于滑膜巨噬细胞上。据报道，原代培养的正常人和类风湿滑膜巨噬细胞表达功能性雄激素受体。我们研究了培养的巨噬细胞代谢雄激素的能力，发现这些细胞能够将睾酮代谢成生物活性代谢物双氢睾酮。因此，巨噬细胞含有类固醇生成的关键酶，特别是5α-还原酶。此外，还分析了在生理浓度的睾酮(10(-8)M)作用下，原代培养的类风湿巨噬细胞产生白细胞介素1β的情况。培养24 h后，条件培养液中IL-1β水平显著下降(p<；0.05)。结论：雄激素可能直接作用于人巨噬细胞，并可能通过受体依赖机制干扰巨噬细胞的某些功能。
THE presence of androgen receptors on synovial macrophages in human normal and rheumatoid synovial tissues has been described previously. It is now reported that primary cultured human macrophages obtained from normal and rheumatoid synovia express functional androgen receptors. We have investigated the capacity of cultured macrophages to metabolize androgens and have found that these cells were capable of metabolizing testosterone to the bioactive metabolite dihydrotestosterone. Therefore, macrophages contain the key enzymes of steroidogenesis, in particular the 5 alpha-reductase. Furthermore, interleukln-1 beta production by primary cultured rheumatoid macrophages was analysed, following exposure to physiological concentrations of testosterone (10(-8) M). A significant decrease of IL-1 beta levels in conditioned media after 24 h (p < 0.05) was observed. It is concluded that androgens may act directly on human macrophages and may interfere with some of their functions via receptor-dependent mechanisms.