Environmental Transmission of the Gut Symbiont Burkholderia to Phloem-Feeding Blissus insularis.
Environmental Transmission of the Gut Symbiont Burkholderia to Phloem-Feeding Blissus insularis.
复制标题
肠道共生体伯克霍尔德里亚向韧皮部喂养的环境传播。
DOI：
10.1371/journal.pone.0161699
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Boucias DG
中科院分区：
文献类型：
--
作者：
Xu Y;Buss EA;Boucias DG
以植物韧皮部为食性的岛叶有专门的中肠隐窝，其中有密集的细胞外细菌共生伯克氏菌群。大多数岛芽胞杆菌在它们的中肠隐窝中携带单一的伯克霍尔德氏菌核型；然而，在宿主人群中存在多样化的伯克霍尔德菌群落。为了了解各种伯克氏菌在岛贝氏杆菌中持续发生的机制及其特定关联，我们研究了潜在的肠道共生体传播途径。PCR扩增检测到成人生殖道内低滴度的伯克氏菌；然而，荧光原位杂交试验未能在这些束中产生可检测的信号。此外，在卵和新生儿中未检测到伯克霍尔德氏菌特异性PCR信号，这表明岛贝氏杆菌不太可能通过卵巢在产前传播肠道共生体。在饲养实验中，用培养的伯克氏菌处理过的圣奥古斯丁草饲养的大多数若虫都携带培养的伯克氏菌菌株。在未经处理的岛白僵菌寄主草中检测到伯克霍尔德菌，并且在未经处理的草上饲养的大多数若虫携带伯克霍尔德菌核型，该核型与植物相关的伯克霍尔德菌菌株密切相关。这些发现表明，尽管不能排除通过卵表面获得伯克氏菌的可能性，但岛小蠊幼虫主要从环境（即植物和土壤）中获得伯克氏菌。此外，我们的研究还解释了如何维持岛屿b种群中伯克霍尔德菌的多样性共生群落。
The plant-phloem-feeding Blissus insularis possesses specialized midgut crypts, which harbor a dense population of the exocellular bacterial symbiont Burkholderia. Most individual B. insularis harbor a single Burkholderia ribotype in their midgut crypts; however, a diverse Burkholderia community exists within a host population. To understand the mechanism underlying the consistent occurrence of various Burkholderia in B. insularis and their specific association, we investigated potential gut symbiont transmission routes. PCR amplification detected a low titer of Burkholderia in adult reproductive tracts; however, fluorescence in situ hybridization assays failed to produce detectable signals in these tracts. Furthermore, no Burkholderia-specific PCR signals were detected in eggs and neonates, suggesting that it is unlikely that B. insularis prenatally transmits gut symbionts via ovarioles. In rearing experiments, most nymphs reared on St. Augustinegrass treated with cultured Burkholderia harbored the cultured Burkholderia strains. Burkholderia was detected in the untreated host grass of B. insularis, and most nymphs reared on untreated grass harbored a Burkholderia ribotype that was closely related to a plant-associated Burkholderia strain. These findings revealed that B. insularis neonates acquired Burkholderia primarily from the environment (i.e., plants and soils), even though the possibility of acquisition via egg surface cannot be excluded. In addition, our study explains how the diverse Burkholderia symbiont community in B. insularis populations can be maintained.