Characterization and drug sensitivity of a novel human ovarian clear cell carcinoma cell line genomically and phenotypically similar to the original tumor.
Characterization and drug sensitivity of a novel human ovarian clear cell carcinoma cell line genomically and phenotypically similar to the original tumor.
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DOI：
10.1002/cam4.1724
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发表时间：
2018-09
期刊：
影响因子：
4
通讯作者：
Curtin NJ
中科院分区：
文献类型：
--
作者：
Franklin M;Gentles L;Matheson E;Bown N;Cross P;Ralte A;Gilkes-Immeson C;Bradbury A;Zanjirband M;Lunec J;Drew Y;O'Donnell R;Curtin NJ
NUCOLL 43是一种新的卵巢透明细胞癌（O-CCC）细胞系，其产生自患者恶性腹水的原代培养物。细胞在细胞培养物中可靠生长，倍增时间约为1000 μ g/ml。45小时，并以高效率形成菌落。它们具有非常高程度的杂合性丢失（洛），影响约85%的基因组，大部分是拷贝中性的，并且几乎与原始肿瘤相同。细胞表达上皮（泛细胞角蛋白）和间充质（波形蛋白）特征，CA 125和p16，就像原始肿瘤一样。它们也表达ARID 1A，但不表达HNF-1β，并且与原始肿瘤一样，p53表达阴性，没有p53功能的证据。NUCOLL 43细胞表达所有其他DNA损伤反应蛋白，并具有功能性同源重组DNA修复。它们对顺铂、PARP抑制剂rucaparib和MDM 2抑制剂不敏感，但对喜树碱、紫杉醇和NVP‐ BEZ 235敏感。NUCOLL 43细胞系代表了O-CCC的一种不同亚型，其为p53和HNF-1β缺失，但表达ARID 1A。它与原始肿瘤在基因组学和蛋白质组学上的高度相似性，以及高水平的洛，使其成为O-CCC研究的一个有趣的细胞系。它已被存放在Ximbio。
NUCOLL43 is a novel ovarian clear cell carcinoma (O‐CCC) cell line that arose from a primary culture of a patient's malignant ascites. The cells grow reliably in cell culture with a doubling time of approx. 45 hours and form colonies at high efficiency. They have a very high degree of loss of heterozygosity (LOH) affecting approximately 85% of the genome, mostly copy neutral and almost identical to the original tumor. The cells express epithelial (pan‐cytokeratin) and mesenchymal (vimentin) characteristics, CA125 and p16, like the original tumor. They also express ARID1A but not HNF‐1β and, like the original tumor, and are negative for p53 expression, with no evidence of p53 function. NUCOLL43 cells express all other DNA damage response proteins investigated and have functional homologous recombination DNA repair. They are insensitive to cisplatin, the PARP inhibitor rucaparib, and MDM2 inhibitors but are sensitive to camptothecin, paclitaxel, and NVP‐BEZ235. The NUCOLL43 cell line represents a distinct subtype of O‐CCC that is p53 and HNF‐1β null but expresses ARID1A. Its high degree of similarity with the original tumor genomically and proteomically, as well as the high level of LOH, make this an interesting cell line for O‐CCC research. It has been deposited with Ximbio.
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影响因子：
5.7
作者：
Ali M;Kamjoo M;Thomas HD;Kyle S;Pavlovska I;Babur M;Telfer BA;Curtin NJ;Williams KJ
通讯作者：
Williams KJ
影响因子：
16.6
作者：
Domcke, Silvia;Sinha, Rileen;Levine, Douglas A.;Sander, Chris;Schultz, Nikolaus
通讯作者：
Schultz, Nikolaus
DOI：
10.1097/pas.0b013e3181cf3d79
发表时间：
2010-03
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Kurman RJ;Shih IeM
通讯作者：
Shih IeM
影响因子：
5.2
作者：
Rundle S;Bradbury A;Drew Y;Curtin NJ
通讯作者：
Curtin NJ
影响因子：
4.7
作者：
Ho, ESC;Lai, CR;Liu, FS
通讯作者：
Liu, FS