Role in pathogenesis of two endo-β-1,4-xylanase genes from the vascular wilt fungus Fusarium oxysporum
Role in pathogenesis of two endo-β-1,4-xylanase genes from the vascular wilt fungus Fusarium oxysporum
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DOI：
10.1006/fgbi.2001.1318
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发表时间：
2002-04-01
影响因子：
3
通讯作者：
Hera, C
中科院分区：
文献类型：
--
作者：
Gómez-Gómez, E;Ruíz-Roldán, MC;Hera, C
从番茄枯萎病菌Fusarium oxysporum f.番茄叶锈菌xyl 4的表达以燕麦斯佩耳特小麦木聚糖为碳源诱导，受到碳分解代谢物的抑制，并优先在碱性环境pH下表达。诱导碳源上xyl 4的转录水平受到氮源性质和浓度的差异调节。RT-PCR结果表明，xyl 4在F.尖孢霉在番茄植物上感染的整个周期期间。目的基因xyl 4和xyl 3的定点失活。oxysporum F.编码家族10木聚糖酶的番茄属植物中的两个基因对番茄植物的毒力没有可检测的影响，表明这两个基因不是致病性所必需的。(C)2002 Elsevler Science（美国）。
A gene, xyl4, whose predicted amino acid sequence shows significant homology with family 11 xylanases, was identified from the tomato vascular wilt fungus Fusarium oxysporum f. sp. lycopersici. Expression of xyl4 is induced on oat spelt xylan as the carbon source, subject to carbon catabolite repression and preferentially expressed at alkaline ambient pH. Transcript levels of xyl4 on an inducing carbon source are differentially regulated by the nature and concentration of the nitrogen source. As shown by RT-PCR, xyl4 is expressed by F. oxysporum during the entire cycle of infection on tomato plants. Targeted inactivation of xyl4 and of xyl3, a previously identified gene of F. oxysporum f. sp. lycopersici encoding a family 10 xylanase, had no detectable effect on virulence on tomato plants, demonstrating that both genes are not essential for pathogenicity. (C) 2002 Elsevler Science (USA).