Cutting edge: The direct action of type IIFN on CD4 T cells is critical for sustaining clonal expansion in response to a viral but not a bacterial infection
Cutting edge: The direct action of type IIFN on CD4 T cells is critical for sustaining clonal expansion in response to a viral but not a bacterial infection
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DOI：
10.4049/jimmunol.176.6.3315
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发表时间：
2006-03-15
影响因子：
4.4
通讯作者：
Murali-Krishna, Kaja
中科院分区：
文献类型：
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作者：
Havenar-Daughton, Colin;Kolumam, Ganesh A.;Murali-Krishna, Kaja
I型干扰素(IFN-I)对APC的作用已被充分研究，但其对CD4T细胞的直接作用尚不清楚。为了解决这一问题，我们将干扰素-I受体缺陷(IFN-IR0)和充足(野生型)TCR转基因的CD4T细胞转移到WT小鼠中，并分析了它们对免疫的反应。在对淋巴细胞性脉络膜脑膜炎病毒免疫的反应中，WT的CD4T细胞扩增了100倍，而干扰素-IR0CD4T细胞扩增了10倍。然而，在单核细胞增多性李斯特氏菌免疫后，WIT和干扰素-IR0的CD4T细胞的扩增幅度接近10倍。淋巴细胞脉络膜脑膜炎病毒免疫后，干扰素-IR0 CD4T细胞扩增不良不是由于增殖缺陷或初始激活，而是子代细胞存活率低。因此，直接的干扰素-I信号可以在CD4T细胞克隆性扩增中发挥关键或最小的作用，具体取决于特定的病原体。
The action of type I IFN (IFN-I) on APCs is well studied, but their direct effect on CD4 T cells is unclear. To address this, we transferred IFN-I receptor-deficient (IFN-IR0) and -sufficient (wild-type, WT) TCR-transgenic CD4 T cells into WT mice and analyzed their response to immunization. In response to lymphocytic choriomeningitis virus immunization, WT CD4 T cells expanded similar to 100-fold, whereas IFN-IR0 CD4 T cells expanded < 10-fold. However, both WIT and IFN-IR0 CD4 T cells expanded similar to 10-fold after Listeria monocytogenes immunization. Poor expansion of IFN-IR0 CD4 T cells after lymphocytic choriomeningitis virus immunization was not due to a defect in proliferation or initial activation but to poor survival of the daughter cells. Thus, direct IFN-I signals can play either a critical or minimal role in CD4 T cell clonal expansion depending on the specific pathogen.