Expression and regulation of the pattern recognition receptors Toll-like receptor-2 and Toll-like receptor-4 in the human placenta
Expression and regulation of the pattern recognition receptors Toll-like receptor-2 and Toll-like receptor-4 in the human placenta
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DOI：
10.1046/j.1365-2567.2002.01491.x
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发表时间：
2002-09-01
期刊：
影响因子：
6.4
通讯作者：
Scheynius, A
中科院分区：
文献类型：
--
作者：
Holmlund, U;Cebers, G;Scheynius, A
胎盘构成了针对入侵感染因子的物理和免疫屏障，并且被认为是先天免疫系统的妊娠特异性组成部分。本研究的目的是调查人类胎盘中 Toll 样受体-2 和 -4（TLR2 和 TLR4）的存在和调节，因为这些受体被认为对于针对病原体的免疫反应很重要。对正常足月妊娠的 28 个胎盘进行免疫组织化学分析，结果显示绒毛和中间滋养层中 TLR2 和 TLR4 具有很强的免疫反应性。通过将足月绒毛膜绒毛外植体与酵母聚糖或脂多糖 (LPS) 一起孵育来评估微生物刺激对 TLR2 和 TLR4 的调节，并使用实时逆转录酶-聚合酶链反应进行分析。酵母聚糖和 LPS 的刺激很容易诱导胎盘培养物中白细胞介素 (IL)-6 和 IL-8 细胞因子的产生，而 TLR2 和 TLR4 mRNA 和蛋白质表达仍保持与未刺激的外植体中相同的高水平。这些数据表明胎儿胎盘单位与微生物相互作用的新机制。
The placenta constitutes a physical and immunological barrier against invading infectious agents and has been suggested to be a pregnancy-specific component of the innate immune system. The aim of this study was to investigate the presence and regulation of Toll-like receptors-2 and -4 (TLR2 and TLR4) in the human placenta, because these receptors are believed to be important for immune responses against pathogens. Twenty-eight placentas from normal term pregnancies were analysed with immunohistochemistry, which showed a strong immunoreactivity for TLR2 and TLR4 in the villous and the intermediate trophoblasts. The regulation of TLR2 and TLR4 by microbial stimulus was assessed by incubating explants of term chorionic villi with zymosan or lipopolysaccharide (LPS) and analysed with real-time reverse transcriptase-polymerase chain reaction. Stimulation with zymosan and LPS readily induced interleukin (IL)-6 and IL-8 cytokine production in the placenta cultures, whereas TLR2 and TLR4 mRNA and protein expression remained at the same high level as in unstimulated explants. These data suggests a novel mechanism for the fetoplacental unit to interact with micro-organisms.