Light and Electron Microscopic Studies of Experimentally-Induced Pathologic Changes in the Rat Periodontal Tissue
Light and Electron Microscopic Studies of Experimentally-Induced Pathologic Changes in the Rat Periodontal Tissue
复制标题
大鼠牙周组织实验诱发病理变化的光和电子显微镜研究
作者：
N. Ijuhin
用光镜和电镜观察Wistar大鼠局部应用脂多糖（LPS）或前列腺素E2 （PGE2）后牙周组织的形态学变化。并对酸性磷酸酶的超微结构和溶菌酶的免疫组化定位进行了研究。计数沿牙槽骨向牙周韧带方向的破骨细胞数量。局部应用LPS后，观察到结膜上皮细胞、牙龈成纤维细胞和肺泡成骨细胞变性坏死，破骨细胞骨吸收增加，成纤维细胞对胶原原纤维的吞噬增强。脂多糖似乎很容易穿过接合上皮进入牙龈结缔组织。在那里，它可以通过激活牙槽骨吸收和增强胶原原纤维的吞噬作用来破坏更深的牙周组织。局部应用PGE2诱导了类似的形态学改变。讨论了PGE2在诱导这些脂多糖相关变化中的可能参与。
Morphological changes in periodontal tissues following topical application of lipopolysaccharide (LPS) or prostaglandin E2 (PGE2) were examined in Wistar rats by light and electron microscopy. Ultrastructural demonstration of acid phosphatase and immunohistochemical localization of lysozyme in these tissues were also studied. The number of osteoclasts along the alveolar bone facing the periodontal ligament was counted. After topical application of LPS, degeneration and necrosis of the junctional epithelial cells, gingival fibroblasts, and alveolar osteoblasts were observed, as were increased bone resorption by osteoclasts and enhanced phagocytosis of collagen fibrils by fibroblasts. LPS appears to traverse easily through the junctional epithelium into the underlying gingival connective tissues. There it can cause destruction of deeper periodontal tissues by activating alveolar bone resorption and by enhancing the phagocytosis of collagen fibrils. PGE2 topically applied induced similar morphological changes. Possible participation of PGE2 in the induction of these LPS-related changes is discussed.
器官培养中细菌内毒素与其他骨吸收刺激物之间的相互作用。
影响因子：
3.5
作者：
Raisz,LG;Nuki,K;Alander,CB;Craig,RG
通讯作者：
Craig,RG