Three-dimensional visualization of forming Hepatitis C virus-like particles by electron-tomography
Three-dimensional visualization of forming Hepatitis C virus-like particles by electron-tomography
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DOI：
10.1016/j.virol.2012.05.001
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发表时间：
2012-09-01
期刊：
影响因子：
3.7
通讯作者：
Abrescia, Nicola G. A.
中科院分区：
文献类型：
--
作者：
Badia-Martinez, Daniel;Peralta, Bibiana;Abrescia, Nicola G. A.
丙型肝炎病毒每年感染近1.7亿人，但人们对其组装途径、结构和包膜蛋白的结构知之甚少。利用昆虫细胞塑料包埋切片的电子断层扫描，我们已经可视化了重组丙型肝炎病毒样颗粒的形态发生。我们的数据提供了病毒在内质网组装的三维草图，显示了不同的出芽阶段和芽的相邻性。后一种现象可能在wt-HCV的组装过程中起重要作用，并解释了其颗粒的大小不均一性。(C) 2012爱思唯尔公司版权所有。
Hepatitis C virus infects almost 170 million people per year but its assembly pathway, architecture and the structures of its envelope proteins are poorly understood. Using electron tomography of plastic-embedded sections of insect cells, we have visualized the morphogenesis of recombinant Hepatitis C virus-like particles. Our data provide a three-dimensional sketch of viral assembly at the endoplasmic reticulum showing different budding stages and contiguity of buds. This latter phenomenon could play an important role during the assembly of wt-HCV and explain the size-heterogeneity of its particles. (C) 2012 Elsevier Inc. All rights reserved.