Control of citrus canker and citrus bacterial spot with bacteriophages
Control of citrus canker and citrus bacterial spot with bacteriophages
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DOI：
10.1094/pdis-92-7-1048
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发表时间：
2008-07-01
期刊：
影响因子：
4.5
通讯作者：
Jones, J. B.
中科院分区：
文献类型：
--
作者：
Balogh, Botond;Canteros, B. I.;Jones, J. B.
研究了噬菌体单独或与铜类杀菌剂联用对柑桔溃疡病和柑桔细菌性斑点病的防治效果。在一组五个温室实验中，噬菌体处理提供了一致的控制柑橘溃疡病，导致疾病严重程度平均降低59%。然而，如果用脱脂乳（一种保护性制剂，其增加噬菌体残余活性）施用，则用噬菌体处理是无效的。在苗圃环境中，噬菌体治疗也减少了疾病，但效果不如铜代森锰锌，一种化学杀虫剂。噬菌体和铜-代森锰锌的整合导致等于或小于单独应用铜-代森锰锌的控制。在商业柑橘苗圃中评估噬菌体处理用于减少由天然接种物引起的柑橘细菌性斑点病。噬菌体治疗提供了显着的疾病减少中度敏感的瓦伦西亚橙在两个试验（48%和35%），然而，对高度敏感的葡萄柚主机是无效的。在实验柑橘苗圃，噬菌体处理提供了显着的控制由噬菌体敏感菌株引起的柑橘细菌性斑点，但同样或不如铜代森锰锌有效。与单独使用铜-代森锰锌相比，噬菌体和铜-代森锰锌的组合没有增加控制。
Bacteriophages, alone or in combination with copper bactericides, were evaluated for managing Asiatic citrus canker and citrus bacterial spot incited by Xanthomonas axonopodis pathovars citri and citrumelo, respectively. In a set of five greenhouse experiments, phage treatment provided consistent control of citrus canker, causing an average of 59% reduction in disease severity. However, treatment with phage was ineffective if applied with skim milk, a protective formulation, which increases phage residual activity. In nursery settings, phage treatment also reduced disease but was less effective than copper-mancozeb, a chemical bactericide. The integration of phage and copper-mancozeb resulted in equal or less control than copper-mancozeb application alone. Phage treatments were evaluated in a commercial citrus nursery for reducing citrus bacterial spot caused by natural inoculum. Phage treatment provided significant disease reduction on moderately sensitive Valencia oranges in two trials (48 and 35%); however, on the highly susceptible grapefruit host it was ineffective. In an experimental citrus nursery, phage treatment provided significant control of citrus bacterial spot caused by a phage-sensitive strain, but was equally or less effective than copper-mancozeb. The combination of phage and copper-mancozeb did not increase control compared with copper-mancozeb alone.