Genomic organization, evolution and functional characterization of soluble toll-like receptor 5 (TLR5S) in miiuy croaker (Miichthys miiuy)
Genomic organization, evolution and functional characterization of soluble toll-like receptor 5 (TLR5S) in miiuy croaker (Miichthys miiuy)
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米氏黄花鱼 (Miichthys miiuy) 可溶性 Toll 样受体 5 (TLR5S) 的基因组组织、进化和功能特征
DOI：
10.1016/j.fsi.2018.05.048
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发表时间：
2018-09-01
影响因子：
4.7
通讯作者：
Xu, Tianjun
中科院分区：
文献类型：
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作者：
Huo, Ruixuan;Zhao, Xueyan;Xu, Tianjun
Toll样受体（TLRs）不仅在天然免疫中，而且在获得性免疫中，在宿主防御病原体入侵中起着关键作用。鱼类TLR种类繁多，特征明显，TLR 5亚家族有两个成员（TLR 5 M和TLR 5S）。然而，TLR 5在鱼类中的确切作用缺乏研究。在这项研究中，TLR 5的可溶性形式（TLR 5S）在Miiuy黄鱼中被鉴定。生物信息学分析表明，小黄鱼TLR 5S缺少跨膜结构域和TIR结构域。也就是说，mmiTLR 5S仅具有富含亮氨酸的重复序列（Leucine-rich repeats，LRR）结构域，这是TLR 5 M与TLR 5S的区别之一。比较基因组分析表明，TLR 5S可能在物种间发生了进化。表达分析表明，mmiTLR 5S在所检测的所有组织中均有表达，并且在哈维氏弧菌感染后12 h，mmiTLR 5S在肝脏和肾脏中的表达显著上调。进一步的功能实验表明，NF-κ B B可被mmiTLR 5S激活，TLR 5S可能在机体免疫应答中起着不可或缺的作用。总之，mmiTLR 5S的研究丰富了TLR 5S的信息，为今后研究硬骨鱼类TLRs系统奠定了基础。
Toll-like receptors (TLRs) play the key role in host defense of invasion of pathogens, not only in the innate immunity, but also in adaptive immunity. There are significant varieties and distinct features in fish TLRs, the TLR5 subfamily have two members (TLR5M and TLR5S). However, the exact role of TLR5 was lack of research in fish. In this study, a soluble form of TLR5 (TLR5S) was identified in miiuy croaker. The bioinformatics analysis showed that miiuy croaker TLR5S lacked the transmembrane domain and TIR domain. In other words, mmiTLR5S only has leucine-rich repeats (LRRs) domain, it is one of differences between TLR5M and TLR5S. Comparative genomic analysis showed that TLR5S might have happened an evolution between species. Expression analysis showed that mmiTLR5S was expressed in all tested miiuy croaker tissues and the mmiTLR5S expressions were significantly upregulated at 12 h in liver and kidney after Vibrio harveyi infection. Further functional experiments showed that NF-kappa B can be actived by mmiTLR5S, TLR5S might be an indispensable role in organism immune response. In short, the study of mmiTLR5S enriches the information of TLR5S and lays the foundation for future research on teleost TLRs system.