Preferential adhesion mediated by Hibris and roughest regulates morphogenesis and patterning in the Drosophila eye
Preferential adhesion mediated by Hibris and roughest regulates morphogenesis and patterning in the Drosophila eye
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DOI：
10.1016/j.devcel.2005.03.011
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发表时间：
2005-06-01
影响因子：
11.8
通讯作者：
Cagan, R
中科院分区：
文献类型：
--
作者：
Bao, SJ;Cagan, R
细胞粘附对于形态发生是必不可少的;然而，细胞粘附协调精确调节的形态发生的机制知之甚少。在这里，我们分析的形态发生过程，组织interrommatidial前体细胞（IPC）的果蝇蛹的眼睛。我们证明，果蝇免疫球蛋白超家族成员Hibris和Roughest是必不可少的IPC在眼睛的形态发生。这两个基因座在互补的细胞类型中表达，Hibris和Roughest蛋白在体内直接结合。初级色素细胞使用Hibris作为组织者在这个过程中; IPC最大限度地减少与相邻IPC的接触，并利用Roughest最大限度地增加与原色的接触。此外，我们提供的证据表明，Hibris和Roughest之间的相互作用促进了连接的形成，并且单个细胞中Roughest的水平决定了它们的竞争能力。我们的研究结果表明，由嗜异性相互作用的细胞粘附分子介导的优先粘附可以通过最小化表面自由能来产生精确的图案。
Cell adhesion is essential for morphogenesis; however, the mechanisms by which cell adhesion coordinates precisely regulated morphogenesis are poorly understood. Here we analyze the morphogenetic processes that organize the interommatidial precursor cells (IPCs) of the Drosophila pupal eye. We demonstrate that the Drosophila immunoglobulin superfamily members Hibris and Roughest are essential for IPC morphogenesis in the eye. The two loci are expressed in complementary cell types, and Hibris and Roughest proteins bind directly in vivo. Primary pigment cells employ Hibris to function as organizers in this process; IPCs minimize contacts with neighboring IPCs and utilize Roughest to maximize contacts with primaries. In addition, we provide evidence that interactions between Hibris and Roughest promote junction formation and that levels of Roughest in individual cells determine their capacity for competition. Our results demonstrate that preferential adhesion mediated by heterophilic interacting cell-adhesion molecules can create a precise pattern by minimizing surface free energy.