Slow growing bacteria survive bacteriophage in isolation.
Slow growing bacteria survive bacteriophage in isolation.
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DOI：
10.1038/s43705-023-00299-5
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发表时间：
2023-09-08
期刊：
影响因子：
--
通讯作者：
Pagliara, Stefano
中科院分区：
文献类型：
--
作者：
Attrill, Erin L;Lapinska, Urszula;Westra, Edze R;Harding, Sarah V;Pagliara, Stefano
细菌与噬菌体之间的相互作用在全球生态系统中具有重要作用；反过来，环境参数的变化又会影响细菌与噬菌体之间的相互作用。然而，对于克隆细菌群体是否具有以不同方式对噬菌体做出反应的不同表型，以及细菌群体中这些表型的丰度是否受到环境参数变化的影响，目前还缺乏了解。在这里，我们研究了营养可获得性、细菌生长速度和噬菌体丰度的变化对噬菌体T4与限制在空间避难所中的单个大肠杆菌细胞之间相互作用的影响。令人惊讶的是，我们发现快速生长的细菌与它们所有的克隆KIN细胞一起存活，而生长缓慢的细菌则在隔离中存活。我们还发现，在分离中存活的细菌数量随着噬菌体剂量的增加而减少，这可能是由于存在二次吸附时的裂解抑制。我们进一步表明，大肠杆菌种群表型组成的这些变化对细菌和噬菌体种群动态有重要影响，因此在研究结构化环境中生态、健康或食品生产环境中细菌与噬菌体的相互作用时，应该考虑这些变化。
The interactions between bacteria and bacteriophage have important roles in the global ecosystem; in turn changes in environmental parameters affect the interactions between bacteria and phage. However, there is a lack of knowledge on whether clonal bacterial populations harbour different phenotypes that respond to phage in distinct ways and whether the abundance of such phenotypes within bacterial populations is affected by variations in environmental parameters. Here we study the impact of variations in nutrient availability, bacterial growth rate and phage abundance on the interactions between the phage T4 and individual Escherichia coli cells confined in spatial refuges. Surprisingly, we found that fast growing bacteria survive together with all of their clonal kin cells, whereas slow growing bacteria survive in isolation. We also discovered that the number of bacteria that survive in isolation decreases at increasing phage doses possibly due to lysis inhibition in the presence of secondary adsorptions. We further show that these changes in the phenotypic composition of the E. coli population have important consequences on the bacterial and phage population dynamics and should therefore be considered when investigating bacteria-phage interactions in ecological, health or food production settings in structured environments.
影响因子：
1.6
作者：
Golec, Piotr;Wiczk, Aleksandra;Majchrzyk, Anna;Los, Joanna M.;Wegrzyn, Grzegorz;Los, Marcin
通讯作者：
Los, Marcin