EC-STM observation on electrochemical response of fluidic phospholipid monolayer on Au(1 1 1) modified with 1-octanethiol
EC-STM observation on electrochemical response of fluidic phospholipid monolayer on Au(1 1 1) modified with 1-octanethiol
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DOI：
10.1016/j.elecom.2006.10.047
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发表时间：
2007-04
影响因子：
5.4
通讯作者：
Soichiro Matsunaga;Ryo Yokomori;D. Ino;Taro Yamada;M. Kawai;Toshihide Kobayshi
中科院分区：
文献类型：
--
作者：
Soichiro Matsunaga;Ryo Yokomori;D. Ino;Taro Yamada;M. Kawai;Toshihide Kobayshi
应用电化学扫描隧道显微镜（EC-STM）观察巯基修饰的金基底上的磷脂层作为模型生物细胞膜。在Au（111）上的单分子层1-辛硫醇上，在中性的0.05 M NH 4ClO 4缓冲溶液中引入一种合成的脂质1，2-二己酰-sn-甘油-3-磷酸胆碱。脂质分子在0.0V（相对于衬底电极电势的RHE）下形成流体层。通过在+0.2V和-0.2V之间循环电极电势，脂质层在流体相和条纹/颗粒状结构之间可逆地改变。这种结构变化可能涉及磷脂的部分分解和寡聚化。这种方法将有助于揭示细胞膜的纳米尺度结构，为分子生物学做出贡献。
Electrochemical scanning tunneling microscopy (EC-STM) was applied to observe phospholipid layers over thiol-modified gold substrates as a model biological cell membrane. On a monolayer of 1-octanethiol on Au (111), a synthetic lipid, 1,2-dihexanoyl-sn-glycero-3-phosphocholine, was introduced in a neutral 0.05M NH4ClO4buffer solution. The lipid molecules formed a fluidic layer at 0.0V vs. RHE of the substrate electrode potential. By cycling the electrode potential between +0.2V and −0.2V, the lipid layer reversibly changed over between the fluidic phase and a striped/grainy structure. This structural change might involve partial decomposition and oligomerization of phospholipids. This method will contribute for molecular biology by revealing the nanometer-scale structure of cell membrane.