Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum
Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum
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DOI：
10.1016/s0168-6496(03)00298-8
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发表时间：
2004-03-15
影响因子：
4.2
通讯作者：
Bolch, CJS
中科院分区：
文献类型：
--
作者：
Green, DH;Llewellyn, LE;Bolch, CJS
链裸藻是一种能产生麻痹性贝类毒素（PST）的甲藻，是温带和热带沃茨麻痹性贝类中毒的主要原因。以往的研究表明，与性休眠期（包囊）表面相关的细菌对G.链状结构本研究旨在对7株不同的G. catenatum产生高和异常低的PST量，长期的目的是了解细菌植物群在开花发展和这种真菌的毒性中的作用。61个细菌分离株进行了培养和遗传学鉴定属于原杆菌（70%），拟杆菌（26%）或放线菌（3%）。就培养的分离物数量而言，α变形菌门是最多的（49%），也是每个G.链状培养从系统发育数据推断的两个表型（功能）性状被证明是存在于每个G.链状培养：首先是能够进行有氧无氧光合作用的α-变形菌，其次是能够利用碳氢化合物和贫营养生长的γ-变形菌。关于甲藻相关细菌自主生产PST的报道，研究了细菌分离株的PST生产，但均未显示产生任何PST样毒素。总之，这项研究已经确定了一些新的趋势，在细菌群落的G。catenatum，反映在其他甲藻的细菌植物群，这可能是特别相关的自然和有害的藻类水华的种群动态。(C)2003年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Gymnodinium catenatum is one of several dinoflagellates that produce a suite of neurotoxins called the paralytic shellfish toxins (PST), responsible for outbreaks of paralytic shellfish poisoning in temperate and tropical waters. Previous research suggested that the bacteria associated with the surface of the sexual resting stages (cyst) were important to the production of PST by G. catenatum. This study sought to characterise the cultivable bacterial diversity of seven different strains of G. catenatum that produce both high and abnormally low amounts of PST, with the long-term aim of understanding the role the bacterial flora has in bloom development and toxicity of this alga. Sixty-one bacterial isolates were cultured and phylogenetically identified as belonging to the Protcobacteria (70%), Bacteroidetes (26%) or Actinobacteria (3%). The Alphaproteobacteria were the most numerous both in terms of the number of isolates cultured (49%) and were also the most abundant type of bacteria in each G. catenatum culture. Two phenotypic (functional) traits inferred from the phylogenetic data were shown to be a common feature of the bacteria present in each G. catenatum culture: firstly, Alphaproteobacteria capable of aerobic anoxygenic photosynthesis, and secondly, Gammaproteobacteria capable of hydrocarbon utilisation and oligotrophic growth. In relation to reports of autonomous production of PST by dinoflagellate-associated bacteria, PST production by bacterial isolates was investigated, but none were shown to produce any PST-like toxins. Overall, this study has identified a number of emergent trends in the bacterial community of G. catenatum which are mirrored in the bacterial flora of other dinoflagellates, and that are likely to be of especial relevance to the population dynamics of natural and harmful algal blooms. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.