Galectin-1 and galectin-3: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population
Galectin-1 and galectin-3: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population
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DOI：
10.1016/j.cca.2014.12.038
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发表时间：
2015-03-10
影响因子：
5
通讯作者：
Das, Satya N.
中科院分区：
文献类型：
--
作者：
Aggarwal, Sadhna;Sharma, Suresh C.;Das, Satya N.
背景：Galectins是一个糖结合蛋白家族，调节细胞的生长、迁移、黏附和凋亡等功能。方法：研究Galectin(Gal)-1和Galectin(Gal)-3在口腔鳞状细胞癌(OSCC)中的表达，并观察其对口腔鳞癌细胞生长和存活的影响。结果：口腔鳞癌患者外周血(P<；0.0001)和肿瘤部位(P<；0.01)中GAL-1和GAL-3的表达水平均显著高于对照组。肿瘤负荷较高的患者这两种Galectins的表达明显高于肿瘤负荷较低的患者。在ROC分析中，血清GAL-1和GAL-3水平分别以4.875和0.871 ng/ml为临界值，区分健康人和患者，敏感性和特异度超过80%。同样，Logistic回归分析显示，过度表达这些蛋白的受试者患口腔鳞癌的风险大约高出3倍。结论：血清GAL-1和GAL-3水平可作为口腔鳞癌的标志物，可用于口腔鳞癌高危人群的筛查。(C)2015爱思唯尔B.V.保留所有权利。
Background: Galectins are a family of carbohydrate binding proteins that regulate several cellular functions such as growth, migration, adhesion and apoptosis.Methods: We investigated the expression of galectin (gal)-1 and galectin (gal)-3 in patients with oral squamous cell carcinoma (OSCC) and observed their effects on growth and survival of OSCC cell lines.Results: OSCC patients expressed significantly higher levels of gal-1 and gal-3 in circulation (p < 0.0001) and at the tumour sites (p < 0.01) as compared to controls. Patients with higher tumour load showed significantly higher expression of both galectins than those with lower tumour load. In ROC analysis, serum levels of gal-1 and gal-3 at cut-off values of 4.875 and 0.871 ng/ml respectively, discriminated between healthy subjects and patients with more than 80% sensitivity and specificity. Similarly, logistic regression analysis revealed about 3-times higher risk of OSCC in subjects over expressing these proteins. Further, exogenous gal-1 and gal-3 significantly increased survival, proliferation and angiogenesis in OSCC cell lines.Conclusions: Serum levels of gal-1 and gal-3 may serve as plausible markers for oral squamous cell carcinoma and may be useful in screening population at a higher risk. (C) 2015 Elsevier B.V. All rights reserved.