Upregulation of TRPC1/6 may be involved in arterial remodeling in rat
Upregulation of TRPC1/6 may be involved in arterial remodeling in rat
复制标题
TRPC1/6 的上调可能参与大鼠动脉重塑。
DOI：
10.1016/j.jss.2014.12.047
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Chen, Liang-Long
中科院分区：
文献类型：
--
作者：
Lin, Xiao-Hong;Hong, Hua-Shan;Chen, Liang-Long
背景：高血压及其并发症与动脉重构有关。瞬时受体电位阳离子通道（TRPC）是调节哺乳动物细胞膜钙稳态的重要非选择性阳离子通道。为探讨TRPC在自发性高血压大鼠（SHR）颈动脉重构中的表达及其与SHR颈动脉重构的关系，将30只雄性SHR随机分为3组，分别于4、8、18 wk处死，并设对照组Wistar-Kyoto大鼠10只。每2 wk测定尾动脉收缩压（SBP）。组织学染色后测定颈动脉重塑参数，包括颈动脉壁厚度（MT）、管腔直径（LD）、中膜面积、胶原面积率和中膜细胞平均核面积。实时聚合酶链反应和免疫印迹分析进行评估TRPC的表达。结果：SHR颈动脉重塑随年龄和收缩压的增加而加重，MT、LD、MT/LD、中膜面积、中膜细胞平均核面积、胶原沉积也随年龄的增加而加重，以18 wk最为明显。有趣的是，TRPC 1，3和6的表达随着年龄和SBP的增加而增加，其中TRPC 1/6在Wistar-Kyoto和18 wk组之间显示出显著差异; TRPC 4/5表达不变，TRPC 7几乎未检测到。结论：TRPC 1和TRPC 6的表达上调可能参与了SHR颈动脉的重构。(C)2015 Elsevier Inc. All rights reserved.
Background: Hypertension and its complications are associated with arterial remodeling. Transient receptor potential cationic channels (TRPCs) are important nonselective cationic channels that regulate calcium homeostasis in mammalian cell membranes. We aimed to study the expression of various TRPC isoforms in spontaneously hypertensive rat (SHR) carotid arterial remodeling and explore the relationship between SHR carotid arterial remodeling and TRPC expression.Materials and methods: Thirty male SHRs were randomly divided into three groups and sacrificed at ages 4, 8, and 18 wk, respectively, with matching control male Wistar-Kyoto rats (n = 10). Caudal artery systolic blood pressure (SBP) was measured every 2 wk. Carotid artery remodeling parameters including carotid artery wall thickness (MT), lumen diameter (LD), medial area, collagen area rate, and average nuclear area in media cells were determined after histologic staining. Real-time polymerase chain reaction and immunoblot assays were performed to assess TRPC expression. Carotid artery remodeling and TRPC expression were reevaluated after ginsenoside Rb1 treatment from eighth to eighteenth week.Results: Carotid artery remodeling of SHRs was aggravated gradually with age and SBP, as well as MT, LD, MT/LD, medial area, average nuclear area in media cells, and collagen deposition, most obvious at 18 wk. Interestingly, expression of TRPC1, 3, and 6 increased with age and SBP, with TRPC1/6 showing significant differences between the Wistar-Kyoto and 18 wk groups; TRPC4/5 expression was unchanged and TRPC7 was barely detected. Importantly, after ginsenoside Rb1 treatment, TRPC1/6 expressions were significantly inhibited, SBP decreased, and the carotid artery remodeling in SHRs relieved.Conclusions: Upregulation of TRPC1 and TRPC6 may be involved in carotid arterial remodeling in SHRs. (C) 2015 Elsevier Inc. All rights reserved.