Untargeted tail acetylation of histones in chromatin: lessons from yeast
Untargeted tail acetylation of histones in chromatin: lessons from yeast
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DOI：
10.1139/o08-097
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发表时间：
2009-02-01
影响因子：
2.9
通讯作者：
Schultz, Michael C.
中科院分区：
文献类型：
--
作者：
Friis, R. Magnus N.;Schultz, Michael C.
核心组蛋白氨基末端赖氨酸残基的动态乙酰化对于核内多种依赖DNA的过程的调控具有重要的功能，包括复制、转录和DNA修复。组蛋白赖氨酸乙酰基酶(KATS)和脱乙酰基酶(HDACs)的靶向和非靶向活性都有助于染色质乙酰化的动力学。虽然历史上靶向乙酰化LRE的机制和功能后果已经被很好地了解，但对于非靶向性组蛋白乙酰化的研究相对较少。在这里，我们回顾了当前对非靶向Kat和HDAC活性调节核小体组蛋白乙酰化状态的机制的理解，重点介绍了在萌芽酵母中对H3和H4的研究结果。我们还强调了这一领域尚未解决的问题，包括在缺乏指导KATS和HDAC活性的顺式依赖机制的情况下如何设定特定的非靶向乙酰化水平的问题。
Dynamic acetylation of lysine residues in the amino-terminal tails of the core histones is functionally important for the regulation of diverse DNA-dependent processes in the nucleus, including replication, transcription, and DNA repair. The targeted and untargeted activities of histone lysine acetylases (KATs) and deacetylases (HDACs) both contribute to the dynamics of chromatin acetylation. While the mechanisms and functional consequences of targeted on historic acetylation Lire well understood, relatively little is known about untargeted histone acetylation. Here, we review the current understanding of the mechanisms by which untargeted KAT and HDAC activities modulate the acetylation state of nucleosomal histones, focusing on results obtained for H3 and H4 in budding yeast. We also highlight unresolved problems in this area, including the question of how a particular steady-state level of untargeted acetylation is set in the absence of cis-dependent mechanisms that instruct the activity of KATs and HDACs.