Helicobacter rodentium sp. nov., a urease-negative Helicobacter species isolated from laboratory mice.
Helicobacter rodentium sp. nov., a urease-negative Helicobacter species isolated from laboratory mice.
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DOI：
10.1099/00207713-47-3-627
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发表时间：
1997-07
期刊：
影响因子：
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通讯作者：
Zeli Shen;James G. Fox;F. Dewhirst;B. J. Paster;C. Foltz;L. Yan;Brian D. Shames;L. Perry
中科院分区：
文献类型：
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作者：
Zeli Shen;James G. Fox;F. Dewhirst;B. J. Paster;C. Foltz;L. Yan;Brian D. Shames;L. Perry
从实验小鼠肠道中分离到一种螺旋形细菌，具有双极、单根、无鞘鞭毛。该生物生长在37和42摄氏度的微氧和厌氧条件下，不水解尿素，过氧化氢酶和氧化酶呈弱阳性，硝酸盐还原为亚硝酸盐，不水解吲哚乙酸或马尿酸，并耐头孢噻吩和萘啶酸。这是第一个尿素酶阴性的鼠螺杆菌。从肠道中分离出来。此外，鸡白痢螺杆菌和这种细菌在螺杆菌属中是独特的，具有无鞘鞭毛。这种新细菌似乎是正常肠道植物群的一部分;尽管其致病潜力尚不清楚，但这种微生物也从与胆汁螺杆菌共感染的腹泻scid小鼠中分离出来。根据16 S rRNA基因序列分析数据和生化及表型标准，新的生物体被归类为一种新的螺旋体，我们建议将其命名为啮齿螺杆菌。模式菌株为MIT 95-1707（= ATCC 700285）。
A spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the intestines of laboratory mice. The organism grew at 37 and 42 degrees C under microaerobic and anaerobic conditions, did not hydrolyze urea, was weakly positive for catalase and oxidase, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and was resistant to cephalothin and nalidixic acid. This is the first urease-negative, murine Helicobacter spp. isolated from intestines. Also, Helicobacter pullorum and this bacterium are unique among the genus Helicobacter in having nonsheathed flagella. The new bacterium appears to be part of the normal intestinal flora; although its pathogenic potential is unknown, this organism was also isolated from scid mice with diarrhea that were co-infected with Helicobacter bilis. On the basis of 16S rRNA gene sequence analysis data and biochemical and phenotypic criteria, the new organism is classified as a novel helicobacter, for which we propose the name Helicobacter rodentium. The type strain is MIT 95-1707 (= ATCC 700285).