Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system.
Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system.
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用测定系统测量乙醇、泼尼松和阿司匹林对粒细胞粘附的抑制。
DOI：
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发表时间：
1974
影响因子：
158.5
通讯作者：
A. Lentnek
中科院分区：
文献类型：
--
作者：
R. Macgregor;P. Spagnuolo;A. Lentnek
摘要本研究建立了一种简便、快速的体外实验方法，用于研究炎症反应的重要组成部分--粒细胞粘附。肝素化全血通过包装在巴斯德吸管中的尼龙纤维过滤，并计算粘附的粒细胞百分比。从早到晚、饭前饭后以及连续几天的试验变异性小于10%。在体外，全血暴露于浓度为100 - 1000 mg/100 ml的乙醇中，引起了剂量依赖性的粘附显著抑制（16- 87%，p<0.05），但与水杨酸钠（5 - 50 mg/100 ml）或氢化可的松琥珀酸钠（500 - 3000 μg/100 ml）孵育无影响。在所有接受40 mg泼尼松或1.2 g阿司匹林的志愿者中，依从性受损，表明糖皮质激素和水杨酸盐可能通过诱导抑制剂影响体内依从性。水杨酸酯、氢化可的松和乙醇的抗炎作用可能是继发于抗炎作用的。
Abstract A simple, rapid, in vitro assay was developed to study granulocyte adherence, an important component of the inflammatory reaction. Heparinized whole blood is filtered through nylon fiber packed in Pasteur pipettes, and the percentage of granulocytes adhering is calculated. Test variability from morning to evening, before and after meals, and on successive days is less than 10 per cent. In vitro exposure of whole blood to ethanol concentrations varying from 100 to 1000 mg per 100 ml caused a dose-dependent, significant inhibition of adherence (16–87 per cent, p<0.05), but incubation with sodium salicylate (5 to 50 mg per 100 ml) or hydrocortisone sodium succinate (500 to 3000 μg per 100 ml) had no effect. In all volunteers receiving 40 mg of prednisone or 1.2 g of aspirin, impaired adherence developed, suggesting that glucocorticoids and salicylates may affect adherence in vivo by inducing an inhibitor. The anti-inflammatory effect of salicylates, hydrocortisone and ethanol may be secondary to the...