SHORT-CHAIN FATTY-ACIDS IN GERM-FREE-MICE AND RATS
SHORT-CHAIN FATTY-ACIDS IN GERM-FREE-MICE AND RATS
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DOI：
10.1093/jn/116.9.1772
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发表时间：
1986-09-01
影响因子：
4.2
通讯作者：
MIDTVEDT, T
中科院分区：
文献类型：
--
作者：
HOVERSTAD, T;MIDTVEDT, T
短链脂肪酸（SCFAs）分析了来自挪威实验室的10只无菌和6只常规小鼠的小肠和盲肠内容物，来自瑞典实验室的5只无菌大鼠和2只无菌小鼠的盲肠内容物和血清，以及挪威和瑞典的高压灭菌、非纯化的老鼠饮食。平均总SCFA浓度为1020 .mu。Mol /kg盲肠和1010 .mu。mol/kg挪威无菌小鼠小肠；124600 .mu。盲肠中Mol /kg和6250 .mu。Mol /kg常规小鼠小肠；490 .mu。在盲肠和370 .mu。瑞典大鼠血清中mol/L；360 .mu。在盲肠和290 .mu。mol/L；挪威和瑞典的非纯化日粮分别为31.4 mmol/kg和19.5 mmol/kg。在所有无菌动物和饲料样品中，乙酸占总浓度的90%以上，但也存在丙酸、异丁酸、正丁酸、异戊酸和正戊酸的可检测浓度。因此，与传统动物相比，无菌动物肠道内容物中SCFAs的浓度较低，但可测量。这些酸很可能主要来自饮食。
The short-chain fatty acids (SCFAs) have been analyzed in small intestinal and cecal content of 10 germfree and six conventional mice from a Norwegian laboratory, in cecal content and serum of five germfree rats and two germfree mice from a Swedish laboratory and in Norwegian and Swedish autoclaved, nonpurified rodor diets. The mean total SCFA concentration was 1020 .mu.mol/kg in cecum and 1010 .mu.mol/kg in small intestine of Norwegian germfree mice; 124,600 .mu.mol/kg in cecum and 6,250 .mu.mol/kg in small intestine of conventional mice; 490 .mu.mol/kg in cecum and 370 .mu.mol/L in serum of Swedish rats; 360 .mu.mol/kg in cecum and 290 .mu.mol/L in serum of Swedish mice; 31.4 mmol/kg in Norwegian and 19.5 mmol/kg in Swedish nonpurified diets. Acetic acid accounted for more than 90% of the total concentration in all samples from germfree animals and diet, but detectable concentrations of propionic, isobutyric, n-butyric, isovaleric and n-valeric acids were also present. Thus, germfree animals have low, but measurable, concentrations of SCFAs in intestinal content compared to conventional animals. Most probably these acids originate mainly from the diet.