ANTIBODY AVIDITY DETERMINATION BY ELISA USING THIOCYANATE ELUTION
ANTIBODY AVIDITY DETERMINATION BY ELISA USING THIOCYANATE ELUTION
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DOI：
10.1016/0022-1759(86)90268-1
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
HOSKING, CS
中科院分区：
文献类型：
--
作者：
PULLEN, GR;FITZGERALD, MG;HOSKING, CS
ELISA法测定了人抗风疹抗体的抗体亲和度。结果显示抗体水平和亲和力的独立性，并建立了接种后抗体亲和力的模式。开发了一种标准ELISA测定的变体，其中含有与抗原结合的抗体的复制孔暴露于浓度增加的朝向硫氰酸盐离子中。对硫氰酸盐洗脱的抗性被用作贪婪度的度量，并使用代表50%有效抗体结合的指数（亲和力指数）来比较不同的血清。亲和指数的板间变异系数为12.6%，板内变异系数为4.1%。
Antibody avidity of human anti-rubella antibodies has been determined using the chaotropic thiocyanate ion in ELISA assays. Independence of antibody level and affinity was shown and the pattern of post-vaccination antibody affinity established. A variation of the standard ELISA assay was developed where replicate wells containing antibody bound to antigens were exposed to increasing concentrations of the chaotropic thiocyanate ion. Resistance to thiocyanate elution was utilised as the measure of avidity and an index (affinity index) representing 50% of effective antibody binding was used to compare different sera. The interplate coefficient of variation for the affinity index was 12.6% whereas the intraplate coefficient of variation was 4.1%.