Spectrophotometric determination of enoxacin as ion-pairs with bromophenol blue and bromocresol purple in bulk and pharmaceutical dosage form.
Spectrophotometric determination of enoxacin as ion-pairs with bromophenol blue and bromocresol purple in bulk and pharmaceutical dosage form.
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DOI：
10.1016/s0731-7085(02)00105-x
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发表时间：
2002-07
影响因子：
3.4
通讯作者：
İ. Süslü;A. Tamer
中科院分区：
文献类型：
--
作者：
İ. Süslü;A. Tamer
建立了三种简便、准确、灵敏的分光光度法测定依诺沙星的含量。该方法基于将该药物萃取到氯仿中，与磺酞染料如溴酚蓝和溴甲酚紫形成离子对。研究并优化了反应的最佳条件。在412 nm处测定依诺沙星-溴酚蓝和410 nm处测定依诺沙星-溴甲酚紫黄色产物的吸光度。依诺沙星-溴酚蓝的线性范围为2.0-20.0 μg ml− 1，依诺沙星-溴甲酚紫的线性范围为0.77-17.62 μg ml− 1。依诺沙星-溴酚蓝和依诺沙星-溴甲酚紫的检测限分别为0.084 μg ml− 1和0.193 μg ml− 1。通过Job方法发现离子对的组成为1：1。该方法已成功地应用于药物制剂中该药物的测定。并与文献中的分光光度法进行了比较。统计学上未发现差异。
Three simple, accurate and sensitive spectrophotometric methods were developed for determination of enoxacin. The methods based on extraction of this drug into chloroform as ion pairs with sulphonphthalein dyes as bromophenol blue and bromocresol purple. The optimum conditions of the reactions were studied and optimized. The absorbance of yellow products was measured at 412 nm for enoxacin–bromophenol blue and 410 nm for enoxacin–bromocresol purple. Linearity ranges were found to be 2.0–20.0 μg ml−1for enoxacin–bromophenol blue and 0.77–17.62 μg ml−1for enoxacin–bromocresol purple. The detection limits were found to be 0.084 μg ml−1and 0.193 μg ml−1for enoxacin–bromophenol blue and enoxacin–bromocresol purple, respectively. The composition of the ion pairs was found 1:1 by Job's method. The developed methods were applied successfully for the determination of this drug in pharmaceutical preparation. The data obtained by developed methods were compared with the spectrophotometric method in literature. No differences were found statistically.